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Pyruvate Carboxylase antibody

Anti-Pyruvate Carboxylase antibody used in Western Blot (WB). GTX87071
Anti-Pyruvate Carboxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX87071

Cat. No. GTX87071

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Pyruvate Carboxylase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

130 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Pyruvate Carboxylase (357-406).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_10733700

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

PC , PCB , pyruvate carboxylase , Pyruvate Carboxylase

Cellular Localization

Mitochondrion matrix

Background

This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Pyruvate Carboxylase antibody used in Western Blot (WB). GTX87071

GTX87071 WB Image

WB analysis of Jurkat cell lysates using GTX87071 Pyruvate Carboxylase antibody. The lane on the right is blocked with the synthesized peptide.

Anti-Pyruvate Carboxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX87071

GTX87071 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX87071 Pyruvate Carboxylase antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

Application Reference

Shan X et al. Exp Ther Med 2019; 17 (5) : 3509-3517 Edoxaban improves atrial fibrillation and thromboembolism through regulation of the Wnt-β-induced PI3K/ATK-activated protein C system.
Application : WB
Reactivity : Mouse
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399