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Pyruvate Dehydrogenase E1 alpha antibody

KO/KD
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Immunoprecipitation (IP). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in IHC (Whole mount) (IHC-Wm). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in IHC (Whole mount) (IHC-Wm). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX104015

Cat. No. GTX104015

Reference 10

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm, IP, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Pyruvate Dehydrogenase E1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:100-1:500
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

Mouse brain , Rat brain , 293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pyruvate Dehydrogenase E1 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951155

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

pyruvate dehydrogenase E1 alpha 1 subunit , PDHA , PDHAD , PDHCE1A , PHE1A

Cellular Localization

Mitochondrion matrix

Background

The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015

GTX104015 WB Image

KO/KD

Wild-type (WT) and Pyruvate Dehydrogenase E1 alpha knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Immunoprecipitation (IP). GTX104015

GTX104015 IP Image

Immunoprecipitation of Pyruvate Dehydrogenase E1 alpha protein from HepG2 whole cell extracts using 5 μg of Pyruvate Dehydrogenase E1 alpha antibody (GTX104015).
Western blot analysis was performed using Pyruvate Dehydrogenase E1 alpha antibody (GTX104015).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015

GTX104015 WB Image

Sample (20 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX104015 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015

GTX104015 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX104015

GTX104015 IHC-P Image

Pyruvate Dehydrogenase E1 alpha antibody detects PDHA1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104015

GTX104015 ICC/IF Image

Pyruvate Dehydrogenase E1 alpha antibody detects Pyruvate Dehydrogenase E1 alpha protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Pyruvate Dehydrogenase E1 alpha stained by Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015

GTX104015 WB Image

Sample (50 μg of whole cell lysate)
A: Rat brain
10% SDS PAGE
GTX104015 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in IHC (Whole mount) (IHC-Wm). GTX104015

GTX104015 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) at 1:200 dilution.

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in IHC (Whole mount) (IHC-Wm). GTX104015

GTX104015 IHC-Wm Image

Pyruvate Dehydrogenase E1 alpha antibody detects Pyruvate Dehydrogenase E1 alpha protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: Pyruvate Dehydrogenase E1 alpha stained by Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) diluted at 1:100.

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015

GTX104015 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX104015

GTX104015 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) at 1:300 dilution.

REFERENCE

Application Reference

Kim S et al. Sci Rep 2023; 13 (1) : 5665 Disruptive lysosomal-metabolic signaling and neurodevelopmental deficits that precede Purkinje cell loss in a mouse model of Niemann-Pick Type-C disease.
Application : WB, IHC (Free Floating)
Reactivity : Mouse
Kirova DG et al. Dev Cell 2022; 57 (14) : 1712-1727.e9 A ROS-dependent mechanism promotes CDK2 phosphorylation to drive progression through S phase.
Marco Rosina et al. Cell Metab 2022; 34 (4) : 533-548.e12 Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue.
Application : WB
Reactivity : Mouse
Rey T et al. Nat Cell Biol 2020; 22 (10) : 1180-1186 Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.
Zhang R et al. Cell Death Dis 2020; 11 (10) : 885 Selective knockdown of hexokinase 2 in rods leads to age-related photoreceptor degeneration and retinal metabolic remodeling.
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REVIEW

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Application Reference
Kim S et al. Sci Rep 2023; 13 (1):5665 Disruptive lysosomal-metabolic signaling and neurodevelopmental deficits that precede Purkinje cell loss in a mouse model of Niemann-Pick Type-C disease.
Application : WB, IHC (Free Floating)
Reactivity : Mouse
Kirova DG et al. Dev Cell 2022; 57 (14):1712-1727.e9 A ROS-dependent mechanism promotes CDK2 phosphorylation to drive progression through S phase.
Marco Rosina et al. Cell Metab 2022; 34 (4):533-548.e12 Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue.
Application : WB
Reactivity : Mouse
Rey T et al. Nat Cell Biol 2020; 22 (10):1180-1186 Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.
Zhang R et al. Cell Death Dis 2020; 11 (10):885 Selective knockdown of hexokinase 2 in rods leads to age-related photoreceptor degeneration and retinal metabolic remodeling.
Jonus HC et al. Biomed Pharmacother 2020; 121:109648 Thiamine mimetics sulbutiamine and benfotiamine as a nutraceutical approach to anticancer therapy.
Application : WB
Reactivity : Human
Chen Y et al. J Biol Chem 2015; 290 (39):23826-37 Critical Role of the Neonatal Fc Receptor (FcRn) in the Pathogenic Action of Antimitochondrial Autoantibodies Synergizing with Anti-desmoglein Autoantibodies in Pemphigus Vulgaris.
Deng W et al. Endocrinology 2014; 155 (5):1618-28 Protein kinase B (PKB/AKT1) formed signaling complexes with mitochondrial proteins and prevented glycolytic energy dysfunction in cultured cardiomyocytes during ischemia-reperfusion injury.
BRADLEY SCOTT HANBERRY et al. 0 2014; TARGETING THE CANCER METABOLIC PHENOTYPE USING HIGH DOSE VITAMIN B1 THERAPY.
Application : WB
Reactivity : Human
Chao LC et al. J Lipid Res 2012; 53 (12):259567 Skeletal muscle Nur77 expression enhances oxidative metabolism and substrate utilization.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169