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Pyruvate Dehydrogenase E1 alpha antibody

KO/KD Orthogonal
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Immunoprecipitation (IP). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in IHC (Whole mount) (IHC-Wm). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in IHC (Whole mount) (IHC-Wm). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015
Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015

Cat. No. GTX104015

Reference 11

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm, IP, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Pyruvate Dehydrogenase E1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:100-1:500
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

Mouse brain , Rat brain , 293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pyruvate Dehydrogenase E1 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951155

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate dehydrogenase E1 alpha 1 subunit , PDHA , PDHAD , PDHCE1A , PHE1A

Cellular Localization

Mitochondrion matrix

Background

The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015

GTX104015 WB Image

KO/KD

Wild-type (WT) and Pyruvate Dehydrogenase E1 alpha knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Immunoprecipitation (IP). GTX104015

GTX104015 IP Image

Immunoprecipitation of Pyruvate Dehydrogenase E1 alpha protein from HepG2 whole cell extracts using 5 μg of Pyruvate Dehydrogenase E1 alpha antibody (GTX104015).
Western blot analysis was performed using Pyruvate Dehydrogenase E1 alpha antibody (GTX104015).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in IHC (Whole mount) (IHC-Wm). GTX104015

GTX104015 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) at 1:200 dilution.

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in IHC (Whole mount) (IHC-Wm). GTX104015

GTX104015 IHC-Wm Image

Pyruvate Dehydrogenase E1 alpha antibody detects Pyruvate Dehydrogenase E1 alpha protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: Pyruvate Dehydrogenase E1 alpha stained by Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) diluted at 1:100.

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015

GTX104015 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX104015

GTX104015 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) at 1:300 dilution.

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015

GTX104015 WB Image

Sample (20 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX104015 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX104015

GTX104015 IHC-P Image

Pyruvate Dehydrogenase E1 alpha antibody detects PDHA1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104015

GTX104015 ICC/IF Image

Pyruvate Dehydrogenase E1 alpha antibody detects Pyruvate Dehydrogenase E1 alpha protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Pyruvate Dehydrogenase E1 alpha stained by Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015

GTX104015 WB Image

Sample (50 μg of whole cell lysate)
A: Rat brain
10% SDS PAGE
GTX104015 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Pyruvate Dehydrogenase E1 alpha antibody used in Western Blot (WB). GTX104015

GTX104015 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

CM MacLeod et al. PLoS One 2023; 18 (11) : e0294312 Trehalose enhances mitochondria deficits in human NPC1 mutant fibroblasts but disrupts mouse Purkinje cell dendritic growth ex vivo
Kim S et al. Sci Rep 2023; 13 (1) : 5665 Disruptive lysosomal-metabolic signaling and neurodevelopmental deficits that precede Purkinje cell loss in a mouse model of Niemann-Pick Type-C disease.
Application : WB, IHC (Free Floating)
Reactivity : Mouse
Kirova DG et al. Dev Cell 2022; 57 (14) : 1712-1727.e9 A ROS-dependent mechanism promotes CDK2 phosphorylation to drive progression through S phase.
Marco Rosina et al. Cell Metab 2022; 34 (4) : 533-548.e12 Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue.
Application : WB
Reactivity : Mouse
Rey T et al. Nat Cell Biol 2020; 22 (10) : 1180-1186 Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.
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REVIEW

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Application Reference
CM MacLeod et al. PLoS One 2023; 18 (11):e0294312 Trehalose enhances mitochondria deficits in human NPC1 mutant fibroblasts but disrupts mouse Purkinje cell dendritic growth ex vivo
Kim S et al. Sci Rep 2023; 13 (1):5665 Disruptive lysosomal-metabolic signaling and neurodevelopmental deficits that precede Purkinje cell loss in a mouse model of Niemann-Pick Type-C disease.
Application : WB, IHC (Free Floating)
Reactivity : Mouse
Kirova DG et al. Dev Cell 2022; 57 (14):1712-1727.e9 A ROS-dependent mechanism promotes CDK2 phosphorylation to drive progression through S phase.
Marco Rosina et al. Cell Metab 2022; 34 (4):533-548.e12 Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue.
Application : WB
Reactivity : Mouse
Rey T et al. Nat Cell Biol 2020; 22 (10):1180-1186 Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.
Zhang R et al. Cell Death Dis 2020; 11 (10):885 Selective knockdown of hexokinase 2 in rods leads to age-related photoreceptor degeneration and retinal metabolic remodeling.
Jonus HC et al. Biomed Pharmacother 2020; 121:109648 Thiamine mimetics sulbutiamine and benfotiamine as a nutraceutical approach to anticancer therapy.
Application : WB
Reactivity : Human
Chen Y et al. J Biol Chem 2015; 290 (39):23826-37 Critical Role of the Neonatal Fc Receptor (FcRn) in the Pathogenic Action of Antimitochondrial Autoantibodies Synergizing with Anti-desmoglein Autoantibodies in Pemphigus Vulgaris.
Deng W et al. Endocrinology 2014; 155 (5):1618-28 Protein kinase B (PKB/AKT1) formed signaling complexes with mitochondrial proteins and prevented glycolytic energy dysfunction in cultured cardiomyocytes during ischemia-reperfusion injury.
BRADLEY SCOTT HANBERRY et al. 0 2014; TARGETING THE CANCER METABOLIC PHENOTYPE USING HIGH DOSE VITAMIN B1 THERAPY.
Application : WB
Reactivity : Human
Chao LC et al. J Lipid Res 2012; 53 (12):259567 Skeletal muscle Nur77 expression enhances oxidative metabolism and substrate utilization.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169