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Pyruvate Dehydrogenase E1 alpha (phospho Ser293) antibody [GT1301]

Recombinant antibody Orthogonal
Anti-Pyruvate Dehydrogenase E1 alpha (phospho Ser293) antibody [GT1301] used in Western Blot (WB). GTX03213
Anti-Pyruvate Dehydrogenase E1 alpha (phospho Ser293) antibody [GT1301] used in Western Blot (WB). GTX03213
Anti-Pyruvate Dehydrogenase E1 alpha (phospho Ser293) antibody [GT1301] used in Western Blot (WB). GTX03213
Anti-Pyruvate Dehydrogenase E1 alpha (phospho Ser293) antibody [GT1301] used in Western Blot (WB). GTX03213

Cat. No. GTX03213

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1301

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all Pyruvate Dehydrogenase E1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S293 of human PDHA1.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate dehydrogenase E1 alpha 1 subunit , PDHA , PDHAD , PDHCE1A , PHE1A

Cellular Localization

Mitochondrion matrix

Background

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-Pyruvate Dehydrogenase E1 alpha (phospho Ser293) antibody [GT1301] used in Western Blot (WB). GTX03213

GTX03213 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pyruvate Dehydrogenase E1 alpha (phospho Ser293) antibody [GT1301] (GTX03213) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Pyruvate Dehydrogenase E1 alpha (phospho Ser293) antibody [GT1301] used in Western Blot (WB). GTX03213

GTX03213 WB Image

Orthogonal

WB analysis of HeLa cells were treated by CIP(20uL/400ul) at 37ºC for 1 hour using GTX03213 Pyruvate Dehydrogenase E1 alpha (phospho Ser293) antibody [GT1301].
Dilution : 1:1000
Loading : 25μg per lane

Anti-Pyruvate Dehydrogenase E1 alpha (phospho Ser293) antibody [GT1301] used in Western Blot (WB). GTX03213

GTX03213 WB Image

Orthogonal

WB analysis of NIH-3T3 cells were treated by CIP(20uL/400ul) at 37ºC for 1 hour using GTX03213 Pyruvate Dehydrogenase E1 alpha (phospho Ser293) antibody [GT1301].
Dilution : 1:1000
Loading : 25μg per lane

Anti-Pyruvate Dehydrogenase E1 alpha (phospho Ser293) antibody [GT1301] used in Western Blot (WB). GTX03213

GTX03213 WB Image

Orthogonal

WB analysis of C6 cells were treated by CIP(20uL/400ul) at 37ºC for 1 hour using GTX03213 Pyruvate Dehydrogenase E1 alpha (phospho Ser293) antibody [GT1301].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 419