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Pyruvate Dehydrogenase E1 beta subunit antibody, N-term

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody, N-term used in Western Blot (WB). GTX46256
Anti-Pyruvate Dehydrogenase E1 beta subunit antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX46256

Cat. No. GTX46256

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
50 μg ($399)
See all Pyruvate Dehydrogenase E1 beta subunit products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

human fetal heart

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human Pyruvate Dehydrogenase E1 beta subunit.

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate dehydrogenase E1 beta subunit , PDHBD , PDHE1-B , PDHE1B , PHE1B

Cellular Localization

Mitochondrion matrix

Background

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody, N-term used in Western Blot (WB). GTX46256

GTX46256 WB Image

WB analysis of human fetal heart tissue using GTX46256 PDHB antibody at 0.2-1μg/ml.

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX46256

GTX46256 IHC-P Image

IHC-P analysis of human heart tissue using GTX46256 PDHB antibody at 1:100. Left to right:DAPI, PDHB, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399