GeneTex
  • Country / Location Selection

United States (US)

Pyruvate Dehydrogenase E1 beta subunit antibody

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX104040
Anti-Pyruvate Dehydrogenase E1 beta subunit antibody used in Western Blot (WB). GTX104040
Anti-Pyruvate Dehydrogenase E1 beta subunit antibody used in Western Blot (WB). GTX104040
Anti-Pyruvate Dehydrogenase E1 beta subunit antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104040
Anti-Pyruvate Dehydrogenase E1 beta subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX104040
Anti-Pyruvate Dehydrogenase E1 beta subunit antibody used in Western Blot (WB). GTX104040

Cat No. GTX104040

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , mouse heart , rat heart

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pyruvate Dehydrogenase E1 beta subunit. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

pyruvate dehydrogenase E1 beta subunit , PDHBD , PDHE1-B , PDHE1B , PHE1B

Cellular Localization

Mitochondrion matrix

Database

Research Area

DATA IMAGES
Anti-Pyruvate Dehydrogenase E1 beta subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX104040

GTX104040 IHC-P Image

Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Pyruvate Dehydrogenase E1 beta subunit antibody (GTX104040) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody used in Western Blot (WB). GTX104040

GTX104040 WB Image

Pyruvate Dehydrogenase E1 beta subunit antibody detects PDHB protein by Western blot analysis.
A. 50 µg rat heart lysate/extract
10 % SDS-PAGE
Pyruvate Dehydrogenase E1 beta subunit antibody (GTX104040) dilution: 1:5000

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody used in Western Blot (WB). GTX104040

GTX104040 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: HCT116
10% SDS PAGE
GTX104040 diluted at 1:1000

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104040

GTX104040 ICC/IF Image

Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Pyruvate Dehydrogenase E1 beta subunit protein stained by Pyruvate Dehydrogenase E1 beta subunit antibody (GTX104040) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX104040

GTX104040 IHC-P Image

Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Pyruvate Dehydrogenase E1 beta subunit antibody (GTX104040) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody used in Western Blot (WB). GTX104040

GTX104040 WB Image

Sample (50 ug of whole cell lysate)
A: mouse heart
10% SDS PAGE
GTX104040 diluted at 1:10000

REFERENCE
REVIEW
There are currently no reviews for Pyruvate Dehydrogenase E1 beta subunit antibody (GTX104040). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169