Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Pyruvate Dehydrogenase E1 beta subunit antibody (GTX104040) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pyruvate Dehydrogenase E1 beta subunit antibody (GTX104040) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Sample (50 ug of whole cell lysate)
A: mouse heart
10% SDS PAGE
GTX104040 diluted at 1:10000

Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Pyruvate Dehydrogenase E1 beta subunit protein stained by Pyruvate Dehydrogenase E1 beta subunit antibody (GTX104040) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Pyruvate Dehydrogenase E1 beta subunit antibody detects PDHB protein by Western blot analysis.
A. 50 μg rat heart lysate/extract
10 % SDS-PAGE
Pyruvate Dehydrogenase E1 beta subunit antibody (GTX104040) dilution: 1:5000

Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Pyruvate Dehydrogenase E1 beta subunit antibody (GTX104040) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: HCT116
10% SDS PAGE
GTX104040 diluted at 1:1000