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Pyruvate Dehydrogenase antibody [3H2-F8-B5]

Anti-Pyruvate Dehydrogenase antibody [3H2-F8-B5] used in Western Blot (WB). GTX49178
Anti-Pyruvate Dehydrogenase antibody [3H2-F8-B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49178

Cat No. GTX49178

Host

Mouse

Clonality

Monoclonal

Clone Name

3H2-F8-B5

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli.

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

PDHCE1A Antibody , pyruvate dehydrogenase (lipoamide) alpha 1 Antibody , PDHA1 Antibody , PDHA Antibody , PHE1A Antibody

Cellular Localization

Mitochondrion matrix

Background

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Research Area

DATA IMAGES

Anti-Pyruvate Dehydrogenase antibody [3H2-F8-B5] used in Western Blot (WB). GTX49178

GTX49178 WB Image

WB analysis of 293, 1299, mESC and Tc1 lysates using Pyruvate Dehydrogenase antibody at a dilution of 1:1,000.

Anti-Pyruvate Dehydrogenase antibody [3H2-F8-B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49178

GTX49178 ICC/IF Image

ICC/IF analysis of HeLa cells using Pyruvate Dehydrogenase antibody at a dilution of 1:150.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf