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Pyruvate Kinase (liver/RBC) antibody [AT1E3]

Anti-Pyruvate Kinase (liver/RBC) antibody [AT1E3] used in Western Blot (WB). GTX57581
Anti-Pyruvate Kinase (liver/RBC) antibody [AT1E3] used in Western Blot (WB). GTX57581
Anti-Pyruvate Kinase (liver/RBC) antibody [AT1E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57581

Cat. No. GTX57581

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT1E3

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Pyruvate Kinase (liver/RBC) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT1E3 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PKLR protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate kinase L/R , PK1 , PKL , PKLR , PKR , PKRL , RPK , pyruvate kinase, liver and RBC , Pyruvate Kinase (liver/RBC)

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Pyruvate Kinase (liver/RBC) antibody [AT1E3] used in Western Blot (WB). GTX57581

GTX57581 WB Image

WB analysis of 293T whole cell lysate using GTX57581 Pyruvate Kinase (liver/RBC) antibody.
Loading : 40 μg
Dilution : 1:500

Anti-Pyruvate Kinase (liver/RBC) antibody [AT1E3] used in Western Blot (WB). GTX57581

GTX57581 WB Image

WB analysis of various samples using GTX57581 Pyruvate Kinase (liver/RBC) antibody.
Lane 1 : Balb/3T3 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Raji whole cell lysate
Lane 4 : K562 whole cell lysate
Lane 5 : A549 whole cell lysate
Loading : 40 μg
Dilution : 1:500

Anti-Pyruvate Kinase (liver/RBC) antibody [AT1E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57581

GTX57581 ICC/IF Image

ICC/IF analysis of Hep3B cells using GTX57581 Pyruvate Kinase (liver/RBC) antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399