GeneTex
United States (US)

Pyruvate Kinase (liver/RBC) antibody

GTX101033_40485_IHC_19011506_445.jpg
GTX101033_40485_WB_R_liver_19011506_594.jpg
GTX101033_40485_WB_19011506_678.jpg
GTX101033_40485_20180727_WB_B_19011506_143.jpg

Cat No. GTX101033

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , rat liver , PKLR-transfected 293T

Specificity/Sensitivity

This antibody is raised against the R-Type isoform. Based on a BLAST result, it may also react with the L-Type isoform.

Predict Reactivity

Mouse, Bovine, Dog, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pyruvate Kinase L/R,Pk1,Pkl,Pkr,Pkrl,Rpk,Pklr

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101033_40485_IHC_19011506_445.jpg

GTX101033 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Pyruvate Kinase (liver/RBC)(GTX101033) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101033_40485_WB_R_liver_19011506_594.jpg

GTX101033 WB Image

Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein by Western blot analysis.
A. 20 ug Rat liver lysate/extract
7.5% SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX101033) dilution: 1:10000

GTX101033_40485_WB_19011506_678.jpg

GTX101033 WB Image

Sample (30 ug of whole cell lysate) 
  A: Jurkat
  B: Raji
  C: K562
  7.5% SDS PAGE 
  GTX101033 diluted at 1:1000

GTX101033_40485_20180727_WB_B_19011506_143.jpg

GTX101033 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Pyruvate Kinase (liver/RBC) antibody (GTX101033) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf