GeneTex
United States (US)

Pyruvate Kinase (liver/RBC) antibody

Overexpression
GTX107546_39911_WB_R_liver_19011506_657.jpg
GTX107546_39911_WB_B_19011506_574.jpg
GTX107546_39911_WB_M_19011506_307.jpg
GTX107546_39911_WB_19011506_519.jpg

Cat No. GTX107546

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

K562 , Raw264.7 , rat liver , human PKLR-transfected 293T

Specificity/Sensitivity

This antibody is raised against the R-Type isoform. Based on a BLAST result, it may also react with the L-Type isoform.

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pyruvate Kinase L/R,Pk1,Pkl,Pkr,Pkrl,Rpk,Pklr

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107546_39911_WB_R_liver_19011506_657.jpg

GTX107546 WB Image

Pyruvate Kinase (liver/RBC) antibody detects PKLR protein by Western blot analysis.
A. 50 µg rat liver lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX107546) dilution: 1:500

GTX107546_39911_WB_B_19011506_574.jpg

GTX107546 WB Image

Overexpression

Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 5 μg whole cell lysate/extract of human PKLR-transfected 293T cells
7.5 % SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX107546) dilution: 1:10000

GTX107546_39911_WB_M_19011506_307.jpg

GTX107546 WB Image

Pyruvate Kinase (liver/RBC) antibody detects PKLR protein by Western blot analysis.
A. 30 µg Raw264.7 whole cell lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX107546) dilution: 1:1000

GTX107546_39911_WB_19011506_519.jpg

GTX107546 WB Image

Pyruvate Kinase (liver/RBC) antibody detects PKLR protein by Western blot analysis.
A. 30 µg K562 whole cell lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX107546) dilution: 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf