APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
Rat liver , Jurkat , Raji , K562 , THP-1 , NCI-H929 , mouse liver
Product Note
This antibody is raised against the R-Type isoform. Based on a BLAST result, it may also react with the L-Type isoform.
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.15 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951261
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
pyruvate kinase L/R , PK1 , PKL , PKRL , RPK
Background
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX107873 WB Image
Pyruvate Kinase (liver/RBC) antibody detects PKLR protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX107873) dilution: 1:500
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GTX107873 WB Image
Pyruvate Kinase (liver/RBC) antibody detects PKLR protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg K562 whole cell lysate/extract
D. 30 μg THP-1 whole cell lysate/extract
E. 30 μg NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX107873) dilution: 1:1000
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GTX107873 WB Image
Pyruvate Kinase (liver/RBC) antibody detects PKLR protein by Western blot analysis.
A. 50 μg Rat liver lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX107873) dilution: 1:500
|
REFERENCE
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REVIEW
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