GeneTex
United States (US)

Pyruvate Kinase (liver/RBC) antibody

GTX107873_39932_WB_R_liver_19011507_440.jpg
GTX107873_39932_WB_19011507_895.jpg
GTX107873_39932_WB_M_liver_19011507_326.jpg

Cat No. GTX107873

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , THP-1 , NCI-H929 , mouse liver , rat liver

Specificity/Sensitivity

This antibody is raised against the R-Type isoform. Based on a BLAST result, it may also react with the L-Type isoform.

Predict Reactivity

Bovine, Dog, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pyruvate Kinase L/R,Pk1,Pkl,Pkr,Pkrl,Rpk,Pklr

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107873_39932_WB_R_liver_19011507_440.jpg

GTX107873 WB Image

Pyruvate Kinase (liver/RBC) antibody detects PKLR protein by Western blot analysis.
A. 50 µg Rat liver lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX107873) dilution: 1:500

GTX107873_39932_WB_19011507_895.jpg

GTX107873 WB Image

Pyruvate Kinase (liver/RBC) antibody detects PKLR protein by Western blot analysis.
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
C. 30 µg K562 whole cell lysate/extract
D. 30 µg THP-1 whole cell lysate/extract
E. 30 µg NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX107873) dilution: 1:1000

GTX107873_39932_WB_M_liver_19011507_326.jpg

GTX107873 WB Image

Pyruvate Kinase (liver/RBC) antibody detects PKLR protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX107873) dilution: 1:500

SDS
Glycerol.pdf
Thimerosal.pdf