GeneTex

  • Country / Location Selection

United States (US)

Pyruvate Kinase (liver/RBC) antibody

Overexpression
Anti-Pyruvate Kinase (liver/RBC) antibody used in IHC (Paraffin sections) (IHC-P). GTX111536
Anti-Pyruvate Kinase (liver/RBC) antibody used in Western Blot (WB). GTX111536
Anti-Pyruvate Kinase (liver/RBC) antibody used in Western Blot (WB). GTX111536
Anti-Pyruvate Kinase (liver/RBC) antibody used in IHC (Paraffin sections) (IHC-P). GTX111536
Anti-Pyruvate Kinase (liver/RBC) antibody used in Western Blot (WB). GTX111536
Anti-Pyruvate Kinase (liver/RBC) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111536

Cat. No. GTX111536

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Pyruvate Kinase (liver/RBC) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , Rat liver , PKLR-transfected 293T

Product Note

This antibody is raised against the R-Type isoform. Based on a BLAST result, it may also react with the L-Type isoform.

Predict Reactivity

Mouse, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951258

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate kinase L/R , PK1 , PKL , PKRL , RPK

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Pyruvate Kinase (liver/RBC) antibody used in IHC (Paraffin sections) (IHC-P). GTX111536

GTX111536 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Pyruvate Kinase (liver/RBC)(GTX111536) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Pyruvate Kinase (liver/RBC) antibody used in Western Blot (WB). GTX111536

GTX111536 WB Image

Sample (30 ug of whole cell lysate) 
  A: 293T
  B: A431
  C: HeLa
  D: HepG2
  E: A375
  7.5% SDS PAGE 
  GTX111536 diluted at 1:1000

Anti-Pyruvate Kinase (liver/RBC) antibody used in Western Blot (WB). GTX111536

GTX111536 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Pyruvate Kinase (liver/RBC) antibody (GTX111536) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Pyruvate Kinase (liver/RBC) antibody used in IHC (Paraffin sections) (IHC-P). GTX111536

GTX111536 IHC-P Image

Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Pyruvate Kinase (liver/RBC) antibody (GTX111536) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Pyruvate Kinase (liver/RBC) antibody used in Western Blot (WB). GTX111536

GTX111536 WB Image

Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein by Western blot analysis.
A. 20 ug Rat liver lysate/extract
7.5% SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX111536) dilution: 1:10000

Anti-Pyruvate Kinase (liver/RBC) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111536

GTX111536 ICC/IF Image

Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Pyruvate Kinase (liver/RBC) protein stained by Pyruvate Kinase (liver/RBC) antibody (GTX111536) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Shimada M et al. Nutr Res 2017; 47 : 21-27 Dietary supplementation with myo-inositol reduces hepatic triglyceride accumulation and expression of both fructolytic and lipogenic genes in rats fed a high-fructose diet.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

There are currently no reviews for Pyruvate Kinase (liver/RBC) antibody (GTX111536). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169