Pyruvate Kinase (liver/RBC) antibody

Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Pyruvate Kinase (liver/RBC) protein stained by Pyruvate Kinase (liver/RBC) antibody (GTX111536) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein by Western blot analysis.
A. 20 ug Rat liver lysate/extract
7.5% SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX111536) dilution: 1:10000

Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Pyruvate Kinase (liver/RBC) antibody (GTX111536) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Pyruvate Kinase (liver/RBC) antibody (GTX111536) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate) 
  A: 293T
  B: A431
  C: HeLa
  D: HepG2
  E: A375
  7.5% SDS PAGE 
  GTX111536 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Pyruvate Kinase (liver/RBC)(GTX111536) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min