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Pyruvate dehydrogenase kinase 1 / PDK1 antibody [4A11]

Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [4A11] used in IHC (Paraffin sections) (IHC-P). GTX60386
Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [4A11] used in Western Blot (WB). GTX60386
Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [4A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60386
Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [4A11] used in Flow cytometry (FACS). GTX60386

Cat. No. GTX60386

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4A11

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all Pyruvate dehydrogenase kinase 1 / PDK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PDK1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate dehydrogenase kinase 1

Cellular Localization

Mitochondrion matrix

Background

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]

Database

Research Area

DATA IMAGES

Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [4A11] used in IHC (Paraffin sections) (IHC-P). GTX60386

GTX60386 IHC-P Image

IHC-P analysis of breast cancer tissue (left) and brain tissue (right) using GTX60386 PDK1 antibody [4A11].

Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [4A11] used in Western Blot (WB). GTX60386

GTX60386 WB Image

WB analysis of NIH3T3 (1), HeLa (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate using GTX60386 PDK1 antibody [4A11].

Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [4A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60386

GTX60386 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60386 PDK1 antibody [4A11].
Green : PDK1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [4A11] used in Flow cytometry (FACS). GTX60386

GTX60386 FACS Image

FACS analysis of Lovo cells using GTX60386 PDK1 antibody [4A11].
Green : PDK1
Purple : negative control

REFERENCE

Application Reference

Sharma R et al. J Exp Clin Cancer Res 2021; 40 (1) : 186 Determinants of resistance to VEGF-TKI and immune checkpoint inhibitors in metastatic renal cell carcinoma.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399