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Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30]

Recombinant antibody
Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30] used in Western Blot (WB). GTX01020
Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30] used in IHC (Paraffin sections) (IHC-P). GTX01020
Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30] used in IHC (Paraffin sections) (IHC-P). GTX01020
Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30] used in Flow cytometry (FACS). GTX01020
Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01020
Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01020

Cat No. GTX01020

Host

Rabbit

Clonality

Monoclonal

Clone Name

JA67-30

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Rat
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

pyruvate dehydrogenase kinase 1,PDK1

Cellular Localization

Mitochondrion matrix

Background

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]

Database

Research Area

DATA IMAGES

Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30] used in Western Blot (WB). GTX01020

GTX01020 WB Image

WB analysis of rat heart tissue lysate using GTX01020 Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30].
Dilution : 1:1000

Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30] used in IHC (Paraffin sections) (IHC-P). GTX01020

GTX01020 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX01020 Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30].

Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30] used in IHC (Paraffin sections) (IHC-P). GTX01020

GTX01020 IHC-P Image

IHC-P analysis of mouse heart tissue section using GTX01020 Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30].

Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30] used in Flow cytometry (FACS). GTX01020

GTX01020 FACS Image

FACS analysis of NIH-3T3 cells using GTX01020 Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30].
Red : primary antibody
Black : unlabelled control
Dilution : 1:100

Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01020

GTX01020 ICC/IF Image

ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX01020 Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01020

GTX01020 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX01020 Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

REFERENCE

There are currently no references for Pyruvate dehydrogenase kinase 1 / PDK1 antibody [JA67-30] (GTX01020). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 369