GeneTex
United States (US)

Pyruvate dehydrogenase kinase 1 / PDK1 antibody [N1C3]

Overexpression
GTX105999_WB_19011506_954.jpg
GTX105999_39953_WB_B_19011506_441.jpg

Cat No. GTX105999

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

A431 , HeLaS3 , Molt-4

Specificity/Sensitivity

Based on sequence homology, this antibody may cross react with other PDK family members.

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pyruvate Dehydrogenase Kinase 1,Pdk1

Cellular Localization

Mitochondrion matrix

Background

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105999_WB_19011506_954.jpg

GTX105999 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
B:MOLT4 (GTX27912)
10% SDS PAGE
GTX105999 diluted at 1:1000

GTX105999_39953_WB_B_19011506_441.jpg

GTX105999 WB Image

Overexpression

PDK1 antibody [N1C3] detects PDK1 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human PDK1 transfected 293T cells
10 % SDS-PAGE
PDK1 antibody [N1C3] (GTX105999) dilution: 1:10000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169