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Pyruvate dehydrogenase kinase 1 / PDK1 antibody [N1C3]

Overexpression
Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [N1C3] used in Western Blot (WB). GTX105999
Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [N1C3] used in Western Blot (WB). GTX105999

Cat. No. GTX105999

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Pyruvate dehydrogenase kinase 1 / PDK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

HeLaS3 , Molt-4 , human PDK1 transfected 293T cells

Product Note

Based on sequence homology, this antibody may cross react with other PDK family members.

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pyruvate dehydrogenase kinase 1 / PDK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951157

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate dehydrogenase kinase 1

Cellular Localization

Mitochondrion matrix

Background

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [N1C3] used in Western Blot (WB). GTX105999

GTX105999 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
B:MOLT4 (GTX27912)
10% SDS PAGE
GTX105999 diluted at 1:1000

Anti-Pyruvate dehydrogenase kinase 1 / PDK1 antibody [N1C3] used in Western Blot (WB). GTX105999

GTX105999 WB Image

Overexpression

PDK1 antibody [N1C3] detects PDK1 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human PDK1 transfected 293T cells
10 % SDS-PAGE
PDK1 antibody [N1C3] (GTX105999) dilution: 1:10000

REFERENCE

Application Reference

Elisa De Tomi et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169