APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A549
Predict Reactivity
Mouse, Rat, Bovine, Chicken, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.64 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human QARS. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11164533
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutaminyl-tRNA synthetase , GLNRS , MSCCA , PRO2195
Cellular Localization
Cytoplasm
Background
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-QARS antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX108713](/templates/default/js/lightbox/images/loading.gif "Anti-QARS antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX108713")
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GTX108713 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using QARS(GTX108713) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX108713 WB Image
Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX108713 diluted at 1:1000
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REFERENCE
There are currently no references for QARS antibody [N3C2], Internal (GTX108713). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for QARS antibody [N3C2], Internal (GTX108713). Be the first to share your experience with this product.
![Anti-QARS antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX108713](/upload/website/prouct_img/small/GTX108713/GTX108713_40618_IHC_w_23060120_673.webp "Anti-QARS antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX108713")
![Anti-QARS antibody [N3C2], Internal used in Western Blot (WB). GTX108713](/upload/website/prouct_img/small/GTX108713/GTX108713_40618_WB_w_23060120_147.webp "Anti-QARS antibody [N3C2], Internal used in Western Blot (WB). GTX108713")
![Anti-QARS antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX108713](/upload/website/prouct_img/normal/GTX108713/GTX108713_40618_IHC_w_23060120_673.webp "Anti-QARS antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX108713")
![Anti-QARS antibody [N3C2], Internal used in Western Blot (WB). GTX108713](/upload/website/prouct_img/normal/GTX108713/GTX108713_40618_WB_w_23060120_147.webp "Anti-QARS antibody [N3C2], Internal used in Western Blot (WB). GTX108713")