APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , rat brain
Predict Reactivity
Bovine, Cat, Dog, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.99 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human QKI. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10620389
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
QKI, KH domain containing RNA binding , Hqk , QK , QK1 , QK3 , hqkI
Cellular Localization
Nucleus , Cytoplasm
Background
QKI belongs to a family of RNA-binding proteins that have an HNRNPK (MIM 600712) homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1; MIM 602489) and SF1 (MIM 601516) (Chen and Richard, 1998 [PubMed 9671495]). QKI proteins regulate RNA splicing, export of target RNAs from the nucleus, translation of proteins, and RNA stability (Lauriat et al., 2008 [PubMed 17918747]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
![Anti-QKI antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115969](/templates/default/js/lightbox/images/loading.gif "Anti-QKI antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115969")
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GTX115969 ICC/IF Image
QKI antibody [N2C3] detects QKI protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: QKI protein stained by QKI antibody [N2C3] (GTX115969) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX115969 IHC-Fr Image
QKI antibody [N2C3] detects QKI protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: QKI protein stained by QKI antibody [N2C3] (GTX115969) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX115969 IHC-Fr Image
QKI antibody [N2C3] detects QKI protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: QKI protein stained by QKI antibody [N2C3] (GTX115969) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX115969 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with QKI antibody [N2C3] (GTX115969) diluted at 1:1000.
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GTX115969 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX115969 diluted at 1:1000
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GTX115969 IHC-P Image
Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using QKI (GTX115969) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for QKI antibody [N2C3] (GTX115969). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for QKI antibody [N2C3] (GTX115969). Be the first to share your experience with this product.
![Anti-QKI antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115969](/upload/website/prouct_img/small/GTX115969/GTX115969_40289_20161130_IFA_w_23060519_615.webp "Anti-QKI antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115969")
![Anti-QKI antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115969](/upload/website/prouct_img/small/GTX115969/GTX115969_40289_20161012_IHC-Fr_R_w_23060519_544.webp "Anti-QKI antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115969")
![Anti-QKI antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115969](/upload/website/prouct_img/small/GTX115969/GTX115969_40289_20170608_IHC-P_M_w_23060519_432.webp "Anti-QKI antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115969")
![Anti-QKI antibody [N2C3] used in Western Blot (WB). GTX115969](/upload/website/prouct_img/small/GTX115969/GTX115969_40289_20170119_WB_R_brain_w_23060519_712.webp "Anti-QKI antibody [N2C3] used in Western Blot (WB). GTX115969")
![Anti-QKI antibody [N2C3] used in Western Blot (WB). GTX115969](/upload/website/prouct_img/small/GTX115969/GTX115969_WB_w_23060519_267.webp "Anti-QKI antibody [N2C3] used in Western Blot (WB). GTX115969")
![Anti-QKI antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115969](/upload/website/prouct_img/small/GTX115969/GTX115969_40289_IHC_w_23060519_939.webp "Anti-QKI antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115969")
![Anti-QKI antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115969](/upload/website/prouct_img/normal/GTX115969/GTX115969_40289_20161130_IFA_w_23060519_615.webp "Anti-QKI antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115969")
![Anti-QKI antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115969](/upload/website/prouct_img/normal/GTX115969/GTX115969_40289_20161012_IHC-Fr_R_w_23060519_544.webp "Anti-QKI antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115969")
![Anti-QKI antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115969](/upload/website/prouct_img/normal/GTX115969/GTX115969_40289_20170608_IHC-P_M_w_23060519_432.webp "Anti-QKI antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115969")
![Anti-QKI antibody [N2C3] used in Western Blot (WB). GTX115969](/upload/website/prouct_img/normal/GTX115969/GTX115969_40289_20170119_WB_R_brain_w_23060519_712.webp "Anti-QKI antibody [N2C3] used in Western Blot (WB). GTX115969")
![Anti-QKI antibody [N2C3] used in Western Blot (WB). GTX115969](/upload/website/prouct_img/normal/GTX115969/GTX115969_WB_w_23060519_267.webp "Anti-QKI antibody [N2C3] used in Western Blot (WB). GTX115969")
![Anti-QKI antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115969](/upload/website/prouct_img/normal/GTX115969/GTX115969_40289_IHC_w_23060519_939.webp "Anti-QKI antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115969")