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QTRT1 antibody

Anti-QTRT1 antibody used in Western Blot (WB). GTX118778
Anti-QTRT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX118778
Anti-QTRT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118778
Anti-QTRT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX118778

Cat. No. GTX118778

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all QTRT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

NT2D1 , PC-3

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human QTRT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11179046

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

queuine tRNA-ribosyltransferase catalytic subunit 1 , FP3235 , TGT , TGUT

Background

tRNA-guanine transglycosylase (TGT; EC 2.4.2.29) synthesizes queuosine (Q), which is found in tRNAs that recognize NAU and NAC codons, encoding tyr, asn, asp, and his. Prokaryotic TGT is a single protein of 43 kD. In contrast, mammalian TGT appears to be a heterodimer consisting of a 60-kD subunit (USP14; MIM 607274) and a 43-kD catalytic subunit (QTRT1) (Deshpande and Katze, 2001 [PubMed 11255023]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-QTRT1 antibody used in Western Blot (WB). GTX118778

GTX118778 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: PC-3
10% SDS PAGE
GTX118778 diluted at 1:1000

Anti-QTRT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX118778

GTX118778 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using QTRT1 antibody(GTX118778) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-QTRT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118778

GTX118778 ICC/IF Image

QTRT1 antibody detects QTRT1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: QTRT1 protein stained by QTRT1 antibody (GTX118778) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-QTRT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX118778

GTX118778 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse muscle, using QTRT1 antibody(GTX118778) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Rashad S et al. bioRxiv 2024; Translational response to mitochondrial stresses is orchestrated by tRNA modifications.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169