GeneTex
  • Country / Location Selection

United States (US)

RAB11B antibody [N1C3]

Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095
Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095
Anti-RAB11B antibody [N1C3] used in IHC (Frozen sections) (IHC-Fr). GTX119095
Anti-RAB11B antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119095
Anti-RAB11B antibody [N1C3] used in Western Blot (WB). GTX119095
Anti-RAB11B antibody [N1C3] used in Western Blot (WB). GTX119095
Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095
Anti-RAB11B antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119095
Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095
Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095
Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095
Anti-RAB11B antibody [N1C3] used in Western Blot (WB). GTX119095

Cat No. GTX119095

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , Mouse brain , PC-12 , Rat2

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAB11B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619861

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAB11B, member RAS oncogene family , H-YPT3 , NDAGSCW

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side

Background

The Ras superfamily of small GTP-binding proteins, which includes the Ras (see MIM 190020), Ral (see MIM 179550), Rho (see MIM 165390), Rap (see MIM 179520), and Rab (see MIM 179508) families, is involved in controlling a diverse set of essential cellular functions. The Rab family, including RAB11B, appears to play a critical role in regulating exocytotic and endocytotic pathways (summary by Zhu et al., 1994 [PubMed 7811277]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095

GTX119095 IHC-P Image

RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
RAB11B antibody [N1C3] (GTX119095) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095

GTX119095 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RAB11B antibody(GTX119095) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAB11B antibody [N1C3] used in IHC (Frozen sections) (IHC-Fr). GTX119095

GTX119095 IHC-Fr Image

RAB11B antibody [N1C3] detects RAB11B Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-RAB11B antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119095

GTX119095 ICC/IF Image

GRAMD1B antibody [C1C2], Internal detects GRAMD1B protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde at RT for 15 min.
Green: GRAMD1B protein stained by GRAMD1B antibody [C1C2], Internal (GTX119095) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-RAB11B antibody [N1C3] used in Western Blot (WB). GTX119095

GTX119095 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB11B antibody [N1C3] used in Western Blot (WB). GTX119095

GTX119095 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095

GTX119095 IHC-P Image

RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB11B antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119095

GTX119095 ICC/IF Image

RAB11B antibody [N1C3] detects RAB11B protein by immunofluorescent analysis.
Sample: DIV9 rat cortical neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:250.
Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095

GTX119095 IHC-P Image

RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095

GTX119095 IHC-P Image

RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB11B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119095

GTX119095 IHC-P Image

RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB11B antibody [N1C3] used in Western Blot (WB). GTX119095

GTX119095 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for RAB11B antibody [N1C3] (GTX119095). Be the first to share your publications with this product.

REVIEW

RAB11B antibody [N1C3] Cat No. GTX119095

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 09-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:5000, 4°C, 12Hr
Secondary Antibody : GTX213110-01, 1:5000, 27°C, 2Hr
Lane Description : Two groups (compound A -/+) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody (GTX119095) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169