APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Drosophila, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS pH7, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.67 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human RAB11B. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10619861
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
RAB11B, member RAS oncogene family , H-YPT3 , NDAGSCW
Cellular Localization
Cell membrane; Lipid-anchor; Cytoplasmic side
Background
The Ras superfamily of small GTP-binding proteins, which includes the Ras (see MIM 190020), Ral (see MIM 179550), Rho (see MIM 165390), Rap (see MIM 179520), and Rab (see MIM 179508) families, is involved in controlling a diverse set of essential cellular functions. The Rab family, including RAB11B, appears to play a critical role in regulating exocytotic and endocytotic pathways (summary by Zhu et al., 1994 [PubMed 7811277]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX119095 IHC-P Image
RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. RAB11B antibody [N1C3] (GTX119095) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX119095 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RAB11B antibody(GTX119095) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX119095 IHC-Fr Image
RAB11B antibody [N1C3] detects RAB11B Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:250. Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX119095 ICC/IF Image
GRAMD1B antibody [C1C2], Internal detects GRAMD1B protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde at RT for 15 min. Green: GRAMD1B protein stained by GRAMD1B antibody [C1C2], Internal (GTX119095) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX119095 IHC-P Image
RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX119095 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX119095 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX119095 WB Image
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX119095 IHC-P Image
RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX119095 ICC/IF Image
RAB11B antibody [N1C3] detects RAB11B protein by immunofluorescent analysis.Sample: DIV9 rat cortical neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:250.Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX119095 IHC-P Image
RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX119095 IHC-P Image
RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
RAB11B antibody [N1C3]
Cat. No. GTX119095
Rating
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( Average 5 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 09-May-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells
Amount used :
10 μg
Blocking :
5% skim milk in TBST, 27°C, 1Hr
Primary Antibody :
1:5000, 4°C, 12Hr
Secondary Antibody :
GTX213110-01, 1:5000, 27°C, 2Hr
Lane Description :
Two groups (compound A -/+) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody (GTX119095) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.