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RAB1B antibody

Anti-RAB1B antibody used in Western Blot (WB). GTX32825
Anti-RAB1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32825
Anti-RAB1B antibody used in IHC (Paraffin sections) (IHC-P). GTX32825
Anti-RAB1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32825
Anti-RAB1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32825

Cat. No. GTX32825

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all RAB1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Product Note

Based on sequence homology, this antibody may cross react with Rab 1A protein.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 7-201 of human RAB1B (NP_112243.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB1B, member RAS oncogene family

Cellular Localization

Cytoplasm,Membrane

Background

Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).[supplied by OMIM, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-RAB1B antibody used in Western Blot (WB). GTX32825

GTX32825 WB Image

WB analysis of various sample lysates using GTX32825 RAB1B antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RAB1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32825

GTX32825 ICC/IF Image

ICC/IF analysis of C6 cells using GTX32825 RAB1B antibody.
Blue : DAPI
Dilution : 1:100

Anti-RAB1B antibody used in IHC (Paraffin sections) (IHC-P). GTX32825

GTX32825 IHC-P Image

IHC-P analysis of human kidney tissue using GTX32825 RAB1B antibody.
Dilution : 1:100

Anti-RAB1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32825

GTX32825 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX32825 RAB1B antibody.
Blue : DAPI
Dilution : 1:100

Anti-RAB1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32825

GTX32825 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32825 RAB1B antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

Application Reference

Chao TY et al. Microbiol Spectr 2023; 11 (1) : e0436922 Subcellular Distribution of BALF2 and the Role of Rab1 in the Formation of Epstein-Barr Virus Cytoplasmic Assembly Compartment and Virion Release.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399