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RAB20 antibody [N1C3]

Orthogonal
Anti-RAB20 antibody [N1C3] used in Western Blot (WB). GTX119559
Anti-RAB20 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119559
Anti-RAB20 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119559

Cat. No. GTX119559

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RAB20 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

HeLa , HeLa (500 μM CoCl2 treatment for 24 hr and 10 μM MG132 treatment for 2 hr)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAB20. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617706

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB20, member RAS oncogene family

Cellular Localization

Golgi apparatus

Background

Plays a role in apical endocytosis/recycling.

Database

Research Area

DATA IMAGES

Anti-RAB20 antibody [N1C3] used in Western Blot (WB). GTX119559

GTX119559 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB20 antibody [N1C3] (GTX119559) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB20 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119559

GTX119559 IHC-P Image

RAB20 antibody [N1C3] detects RAB20 protein at cytoplasm on human hepatocellular carcinomas by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinomas.
RAB20 antibody [N1C3] (GTX119559) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAB20 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119559

GTX119559 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using RAB20(GTX119559) antibody at 1:200 dilution.

REFERENCE

Application Reference

Schnettger L et al. Cell Host Microbe 2017; 21 (5) : 619-628.e5 A Rab20-Dependent Membrane Trafficking Pathway Controls M.-tuberculosis Replication by Regulating Phagosome Spaciousness and Integrity.
Application : ICC/IF
Reactivity : Mouse
Pei G et al. Mol Biol Cell 2015; 26 (17) : 3061-70 *-γ-inducible rab20 regulates endosomal morphology and egfr degradation in macrophages.
Pei G et al. J Cell Sci 2014; 127 (Pt 9) : 2071-82 Identification of an immune-regulated phagosomal Rab cascade in macrophages.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169