GeneTex
United States (US)

RAB2B antibody

GTX108606_WB_19011507_758.jpg
GTX108606_IFA_19011507_797.jpg
GTX108606_39939_IHC_19011507_283.jpg
GTX108606_39939_WB_M_brain_19011507_940.jpg

Cat No. GTX108606

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

HepG2 , mouse brain

Predict Reactivity

Rat, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human RAB2B Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rab2B, Member Ras Oncogene Family,Rab2B

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side (Potential) , Endoplasmic reticulum membrane (Potential) , Golgi apparatus membrane

Background

Members of the Rab protein family are nontransforming monomeric GTP-binding proteins of the Ras superfamily that contain 4 highly conserved regions involved in GTP binding and hydrolysis. Rab proteins are prenylated, membrane-bound proteins involved in vesicular fusion and trafficking; see MIM 179508.[supplied by OMIM]

Database

DATA IMAGES
GTX108606_WB_19011507_758.jpg

GTX108606 WB Image

Sample(30 ug whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX108606 diluted at 1:500

GTX108606_IFA_19011507_797.jpg

GTX108606 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAB2B(GTX108606) antibody at 1:200 dilution.

GTX108606_39939_IHC_19011507_283.jpg

GTX108606 IHC-P Image

RAB2B antibody detects RAB2B protein at cytoplasm and membrane on human liver carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded liver carcinoma.
RAB2B antibody (GTX108606) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108606_39939_WB_M_brain_19011507_940.jpg

GTX108606 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX108606 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169