GeneTex
  • Country / Location Selection

United States (US)

RAB35 antibody [N1C3]

Anti-RAB35 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120294
Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294
Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294
Anti-RAB35 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX120294
Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294
Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294

Cat No. GTX120294

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Hamster
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

U87-MG , mouse brain , RAB35-transfected 293T , *CHO

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAB35. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724116

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAB35, member RAS oncogene family , H-ray , RAB1C , RAY

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side (Potential) , Melanosome

Database

Research Area

DATA IMAGES

Anti-RAB35 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120294

GTX120294 ICC/IF Image

RAB35 antibody [N1C3] detects RAB35 protein at Golgi apparatus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAB35 stained by RAB35 antibody [N1C3] (GTX120294) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294

GTX120294 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294

GTX120294 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB35 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX120294

GTX120294 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using RAB35(GTX120294) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294

GTX120294 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294

GTX120294 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody (GTX120294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for RAB35 antibody [N1C3] (GTX120294). Be the first to share your publications with this product.

REVIEW

RAB35 antibody [N1C3] Cat No. GTX120294

Rating ( Average 3 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 31-Mar-2014
Score :
Application Tested : ICC/IF
Sample Species : Hm
Sample : Chinese Hamster Ovary Cells
Blocking : 1% BSA in PBS, 37°C, 0.5Hr
Fixation : PFA, 37°C, 0.08Hr
Permeabilization /Antigen retrieval : Triton 0.5%, 37°C, 2minHr
Primary Antibody : 1:30, 37°C, 1Hr
Figure Note : This picture shows a cell in cytokinesis stained with Rab35 antibody. Rab35 (red) localises to midbody.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf