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RAB3C antibody [N1C3]

Anti-RAB3C antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108610
Anti-RAB3C antibody [N1C3] used in Western Blot (WB). GTX108610
Anti-RAB3C antibody [N1C3] used in Western Blot (WB). GTX108610
Anti-RAB3C antibody [N1C3] used in Western Blot (WB). GTX108610
Anti-RAB3C antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX108610

Cat No. GTX108610

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human RAB3C Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951574

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAB3C, member RAS oncogene family

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side

Background

RAB proteins, such as RAB3C, are small GTPases implicated in the regulation of vesicular trafficking between membrane compartments (Cheng et al., 2002 [PubMed 12296628]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-RAB3C antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108610

GTX108610 ICC/IF Image

RAB3C antibody [N1C3] detects RAB3C protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAB3C stained by RAB3C antibody [N1C3] (GTX108610) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RAB3C antibody [N1C3] used in Western Blot (WB). GTX108610

GTX108610 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX108610 diluted at 1:5000

Anti-RAB3C antibody [N1C3] used in Western Blot (WB). GTX108610

GTX108610 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
GTX108610 diluted at 1:1000

Anti-RAB3C antibody [N1C3] used in Western Blot (WB). GTX108610

GTX108610 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB3C antibody [N1C3] (GTX108610) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB3C antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX108610

GTX108610 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using RAB3C(GTX108610) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

RAB3C antibody [N1C3] Cat No. GTX108610

Rating ( Average 5 based on 5 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Cancer in 2017.PMID: 28784136
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Cancer in 2017.PMID: 28784136
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Cancer in 2017.PMID: 28784136
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Cancer in 2017.PMID: 28784136
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Cancer in 2017.PMID: 28784136
SDS
Glycerol.pdf
Thimerosal.pdf