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RAB4A antibody

Comparison
Anti-RAB4A antibody used in Western Blot (WB). GTX108607
Anti-RAB4A antibody used in IHC (Paraffin sections) (IHC-P). GTX108607

Cat. No. GTX108607

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RAB4A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAB4A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951576

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB4A, member RAS oncogene family , HRES-1 , HRES-1/RAB4 , HRES1 , RAB4

Cellular Localization

Membrane; Peripheral membrane protein , Cytoplasm

Background

Protein transport. Probably involved in vesicular traffic.

Database

Research Area

DATA IMAGES

Anti-RAB4A antibody used in Western Blot (WB). GTX108607

GTX108607 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with RAB4A antibody (GTX108607) diluted at 1:500 and competitor's antibody (SC-312) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-RAB4A antibody used in IHC (Paraffin sections) (IHC-P). GTX108607

GTX108607 IHC-P Image

RAB4A antibody detects RAB4A protein at cytoplasm and membrane on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded lung carcinoma.
RAB4A antibody (GTX108607) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Heisler FF et al. Neuron 2018; 99 (6) : 1155-1169.e9 Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169