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RAB5A antibody [HL1497]

Recombinant antibody KO/KD
Anti-RAB5A antibody [HL1497] used in IHC (Paraffin sections) (IHC-P). GTX636971
Anti-RAB5A antibody [HL1497] used in IHC (Paraffin sections) (IHC-P). GTX636971
Anti-RAB5A antibody [HL1497] used in Western Blot (WB). GTX636971
Anti-RAB5A antibody [HL1497] used in IHC (Paraffin sections) (IHC-P). GTX636971
Anti-RAB5A antibody [HL1497] used in IHC (Paraffin sections) (IHC-P). GTX636971

Cat. No. GTX636971

3

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1497

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all RAB5A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human RAB5A. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB5A, member RAS oncogene family , RAB5

Cellular Localization

Cell membrane,Cytoplasmic vesicle,Membrane,Cytoplasm, cytosol,Endosome membrane

Database

Research Area

DATA IMAGES

Anti-RAB5A antibody [HL1497] used in IHC (Paraffin sections) (IHC-P). GTX636971

GTX636971 IHC-P Image

RAB5A antibody [HL1497] detects RAB5A protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
RAB5A stained by RAB5A antibody [HL1497] (GTX636971) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB5A antibody [HL1497] used in IHC (Paraffin sections) (IHC-P). GTX636971

GTX636971 IHC-P Image

RAB5A antibody [HL1497] detects RAB5A protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
RAB5A stained by RAB5A antibody [HL1497] (GTX636971) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB5A antibody [HL1497] used in Western Blot (WB). GTX636971

GTX636971 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB5A antibody [HL1497] (GTX636971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB5A antibody [HL1497] used in IHC (Paraffin sections) (IHC-P). GTX636971

GTX636971 IHC-P Image

RAB5A antibody [HL1497] detects RAB5A protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat lymph node.
RAB5A stained by RAB5A antibody [HL1497] (GTX636971) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB5A antibody [HL1497] used in IHC (Paraffin sections) (IHC-P). GTX636971

GTX636971 IHC-P Image

RAB5A antibody [HL1497] detects RAB5A protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog muscle.
RAB5A stained by RAB5A antibody [HL1497] (GTX636971) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for RAB5A antibody [HL1497] (GTX636971). Be the first to share your publications with this product.
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REVIEW

RAB5A antibody [HL1497] Cat. No. GTX636971

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-RAB5A antibody [HL1497] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636971
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HAP1 WT and RAB5A KO cells
Blocking : PBS, 5% BSA, 5% goat serum, 0.01% Triton X-100, 25°C, 0.5Hr
Permeabilization /Antigen retrieval : PBS, 0.1% Triton X-100, 25°C, 0.17Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : WT cells: Green dashed line KO cells: Magenta dashed line Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-RAB5A antibody [HL1497] used in Immunoprecipitation (IP). GTX636971
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : IP
Sample Species : Hu
Sample : HAP1 cells
Lane Description : SM: 4% starting material UB: 4% unbound fraction
Figure Note : Antibody-bead conjugates were prepared by adding 2.0 μg of antibody to 500 μL of IP Lysis Buffer, together, 30 μL of protein A-Sepharose beads. 0.5 mL aliquots at 2.0 mg/mL of lysate were incubated, an antibody-bead conjugate for ~1 hrs at 4ºC. Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-RAB5A antibody [HL1497] used in Western Blot (WB). GTX636971
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and RAB5A KO cells
Amount used : 30 μg
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
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Package List Price ($)
$ 399
$ 169
Custom Conjugations