RAB6 (Q72L mutant specific) antibody [AA2]

Cat. No. GTX33942

Host	Human
Clonality	Monoclonal
Clone Name	AA2
Isotype	IgG2
Application	ICC/IF
Reactivity	Human, Mouse, Drosophila
Package	100 μg ($439)

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Datasheet
Document

View product citations for antibody GTX33942 on CiteAb

APPLICATION

Application Note

Immunocytochemistry: 1:1000

Product Note

Recognizes human, mouse and Drosophila GTP-bound Rab6a and Rab6b and mutant Rab6Q72L. Does not detect Rab6-GDP.

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Rab6AQ72L, a GTP-locked mutant of Rab6A in which Gln72 is replaced by Leu.

Purification

Protein A-affinity purified.

Purity

??95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX33942 Image

GTX33942 ICC/IF Image

Rab6-GTP is detected by immunocytochemistry using anti-Rab6-GTP, mAb (AA2). Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Saponin 0.05 % and incubated with anti-Rab6-GTP, mAb (AA2) (1μg /ml) in PBS-BSA-Saponin. After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody in PBS-BSA-Saponin for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 439

Qty

RAB6 (Q72L mutant specific) antibody [AA2]

Cat. No. GTX33942

Host

Clonality

Clone Name

Isotype

Application

Reactivity