GeneTex
United States (US)

RAB6 (Q72L mutant specific) antibody [AA2]

GTX33942_20160831_ICCIF_18121410_394.jpg
GTX33942_20160831_Structure_18121410_559.jpg

Cat No. GTX33942

Host Human
Clonality Monoclonal
Clone Name AA2
Isotype IgG2
Application ICC/IF
Reactivity Human, Mouse, Drosophila
APPLICATION

Application Note

Immunocytochemistry: 1:1000

Specificity/Sensitivity

Recognizes human, mouse and Drosophila GTP-bound Rab6a and Rab6b and mutant Rab6Q72L. Does not detect Rab6-GDP.
PROPERTIES

Form

Liquid

Buffer

PBS containing 10% glycerol and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Rab6AQ72L, a GTP-locked mutant of Rab6A in which Gln72 is replaced by Leu.

Purification

Protein A-affinity purified.

Purity

≧95% (SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
GTX33942_20160831_ICCIF_18121410_394.jpg

GTX33942 ICC/IF Image

Rab6-GTP is detected by immunocytochemistry using anti-Rab6-GTP, mAb (AA2). Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Saponin 0.05 % and incubated with anti-Rab6-GTP, mAb (AA2) (1μg /ml) in PBS-BSA-Saponin. After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody in PBS-BSA-Saponin for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.

GTX33942_20160831_Structure_18121410_559.jpg

GTX33942 Image

Structure of the recombinant antibody anti-Rab6 (h)-GTP, mAb (rec.) (AA2). The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human IgG2 Fc region.

Package List Price ($)
$ 379