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RAB6A antibody [HL1047]

Anti-RAB6A antibody [HL1047] used in Western Blot (WB). GTX635981
Anti-RAB6A antibody [HL1047] used in Western Blot (WB). GTX635981
Anti-RAB6A antibody [HL1047] used in Western Blot (WB). GTX635981
Anti-RAB6A antibody [HL1047] used in IHC (Paraffin sections) (IHC-P). GTX635981
Anti-RAB6A antibody [HL1047] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635981
Anti-RAB6A antibody [HL1047] used in Western Blot (WB). GTX635981
Anti-RAB6A antibody [HL1047] used in Western Blot (WB). GTX635981

Cat. No. GTX635981

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1047

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Zebrafish, Drosophila
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human RAB6A recombinant protein.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909932

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB6A, member RAS oncogene family , RAB6

Cellular Localization

Golgi apparatus membrane

Background

This gene encodes a member of the RAB family, which belongs to the small GTPase superfamily. GTPases of the RAB family bind to various effectors to regulate the targeting and fusion of transport carriers to acceptor compartments. This protein is located at the Golgi apparatus, which regulates trafficking in both a retrograde (from early endosomes and Golgi to the endoplasmic reticulum) and an anterograde (from the Golgi to the plasma membrane) directions. Myosin II is an effector of this protein in these processes. This protein is also involved in assembly of human cytomegalovirus (HCMV) by interacting with the cellular protein Bicaudal D1, which interacts with the HCMV virion tegument protein, pp150. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-RAB6A antibody [HL1047] used in Western Blot (WB). GTX635981

GTX635981 WB Image

Wild-type (WT) and RAB6A knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (GTX635981) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB6A antibody [HL1047] used in Western Blot (WB). GTX635981

GTX635981 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (GTX635981) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB6A antibody [HL1047] used in Western Blot (WB). GTX635981

GTX635981 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (GTX635981) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB6A antibody [HL1047] used in IHC (Paraffin sections) (IHC-P). GTX635981

GTX635981 IHC-P Image

RAB6A antibody [HL1047] detects RAB6A protein at Golgi apparatus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
RAB6A stained by RAB6A antibody [HL1047] (GTX635981) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB6A antibody [HL1047] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635981

GTX635981 ICC/IF Image

RAB6A antibody [HL1047] detects RAB6A protein at Golgi apparatus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAB6A stained by RAB6A antibody [HL1047] (GTX635981) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RAB6A antibody [HL1047] used in Western Blot (WB). GTX635981

GTX635981 WB Image

Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (GTX635981) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-RAB6A antibody [HL1047] used in Western Blot (WB). GTX635981

GTX635981 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (GTX635981) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

RAB6A antibody [HL1047] Cat. No. GTX635981

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 08-Jul-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and RAB6A KO cells
Amount used : 40μg
Blocking : 5% milk, 1Hr
Primary Antibody : 1:200, 4°C, >12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Package List Price ($)
$ 399
$ 169
Custom Conjugations