RAB7A antibody [AT10E4]

Cat. No. GTX57709

Host	Mouse
Clonality	Monoclonal
Clone Name	AT10E4
Isotype	IgG2a
Application	WB, ICC/IF, FACS
Reactivity	Human
Package	100 μl ($399)

See all RAB7A products

Datasheet
Document

View product citations for antibody GTX57709 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
FACS	Assay dependent

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT10E4 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human RAB9A protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB7A, member RAS oncogene family , PRO2706 , RAB7 , RAB7A

Cellular Localization

Cytoplasmic vesicle,Phagosome membrane,Late endosome membrane,Lysosome membrane,Melanosome membrane,Autophagosome membrane,Lipid droplet,Endosome membrane

Background

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008]

Database

Gene ID: 7879 RAB7A
UniProt: P51149 RAB7A

Research Area

DATA IMAGES

GTX57709 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57709 RAB7A antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

GTX57709 WB Image

WB analysis of various samples using GTX57709 RAB7A antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : PC-3 whole cell lysate
Lane 5 : 293T whole cell lysate
Lane 6 : SK-OV-3 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

GTX57709 FACS Image

FACS analysis of HeLa cells using GTX57709 RAB7A antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty