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RAB7A antibody [Rab7-117]

Anti-RAB7A antibody [Rab7-117] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16196
Anti-RAB7A antibody [Rab7-117] used in Western Blot (WB). GTX16196

Cat. No. GTX16196

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 Rab7-117

Isotype

 IgG2b

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human, Mouse, Rat, Bovine, Dog, Chicken, Monkey
Package
100 μl ($409)
See all RAB7A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-1 μg/ml
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

HeLa

Product Note

Monoclonal Anti-Rab7 recognizes human, monkey, bovine, rat, mouse, and chicken Rab7.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic peptide corresponding to amino acids 163-177 of human Rab7.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

RRID

AB_2887527

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB7A, member RAS oncogene family , PRO2706 , RAB7

Cellular Localization

Lysosome membrane,Endosome membrane

Database

Research Area

DATA IMAGES

Anti-RAB7A antibody [Rab7-117] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16196

GTX16196 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX16196 RAB7A antibody [Rab7-117] at 10 μg/mL(green).Cells were fixed and permeabilized with 4% PFA followed by 0.5% saponin.

Anti-RAB7A antibody [Rab7-117] used in Western Blot (WB). GTX16196

GTX16196 WB Image

WB analysis of (1)HeLa (2) HepG2 (3) NRK (4)3T3 (5) MDCK cells using GTX16196 RAB7A antibody [Rab7-117] at 1 μg/mL.

REFERENCE

Application Reference

Shi Y et al. Nat Med 2018; (Epub) Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.
Application : ICC/IF
Reactivity : Human
O'Rourke JG et al. Science 2016; 351 (6279) : 1324-9 C9orf72 is required for proper macrophage and microglial function in mice.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409