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RAB7A antibody

Anti-RAB7A antibody used in Western Blot (WB). GTX02417
Anti-RAB7A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02417

Cat No. GTX02417

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:1500-1:50000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2 μm filtered PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to the C-terminus of the Human RAB7A / Rab-7a

Purification

Purified by Protein A and Antigen Affinity

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAB7A, member RAS oncogene family , PRO2706 , RAB7

Cellular Localization

Lysosome membrane , Endosome membrane

Background

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RAB7A antibody used in Western Blot (WB). GTX02417

GTX02417 WB Image

WB analysis of A431 whole cell lysate (Lane A) using GTX02417 RAB7A antibody.
Dilution : 1:500
Loading : 30 μg

Anti-RAB7A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02417

GTX02417 ICC/IF Image

ICC/IF analysis of PFA-fixed A431 cells using GTX02417 RAB7A antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:5000
Permeabilzation : 0.3% Triton X-100

REFERENCE

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REVIEW

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