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RAB7A antibody

Anti-RAB7A antibody used in Western Blot (WB). GTX130847
Anti-RAB7A antibody used in Western Blot (WB). GTX130847
Anti-RAB7A antibody used in IHC (Paraffin sections) (IHC-P). GTX130847
Anti-RAB7A antibody used in IHC (Paraffin sections) (IHC-P). GTX130847
Anti-RAB7A antibody used in Western Blot (WB). GTX130847

Cat No. GTX130847

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Dog, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAB7A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886362

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAB7A, member RAS oncogene family , PRO2706 , RAB7

Cellular Localization

Late endosome , Lysosome , Cytoplasmic vesicle , phagosome , Melanosome

Background

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RAB7A antibody used in Western Blot (WB). GTX130847

GTX130847 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB7A antibody (GTX130847) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-RAB7A antibody used in Western Blot (WB). GTX130847

GTX130847 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB7A antibody (GTX130847) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-RAB7A antibody used in IHC (Paraffin sections) (IHC-P). GTX130847

GTX130847 IHC-P Image

RAB7A antibody detects RAB7A protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
RAB7A antibody (GTX130847) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB7A antibody used in IHC (Paraffin sections) (IHC-P). GTX130847

GTX130847 IHC-P Image

RAB7A antibody detects RAB7A protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
RAB7A antibody (GTX130847) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB7A antibody used in Western Blot (WB). GTX130847

GTX130847 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with RAB7A antibody (GTX130847) diluted at 1:500 and competitor's antibody (sc-10767) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf