GeneTex
United States (US)

RAB7A antibody

GTX132548_42452_20161027_WB_competitor_watermark_18121410_913.jpg
GTX132548_42452_20181221_WB_shRNA_watermark_19011519_186.jpg
GTX132548_42452_20160614_IFA_18121410_829.jpg
GTX132548_42452_20160428_WB_18121410_529.jpg
GTX132548_42452_20171115_ICC_IF_R_18121410_331.jpg
GTX132548_42452_20160601_IHC-P_M_18121410_892.jpg
GTX132548_42452_20160601_IHC-P_R_18121410_700.jpg

Cat No. GTX132548

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , RAB7A shRNA-transfected 293T

Predict Reactivity

Sheep, Bovine, Dog, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Rab7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PRO2706 antibody , RAB7A antibody , RAB7A, member RAS oncogene family antibody , Rab7 antibody , Rab7 antibody

Cellular Localization

Late endosome , Lysosome , Cytoplasmic vesicle , phagosome , Melanosome

Background

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX132548_42452_20161027_WB_competitor_watermark_18121410_913.jpg

GTX132548 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB7A antibody (GTX132548) diluted at 1:1000 and competitor's antibody (SC-10767) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX132548_42452_20181221_WB_shRNA_watermark_19011519_186.jpg

GTX132548 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB7A antibody (GTX132548) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX132548_42452_20160614_IFA_18121410_829.jpg

GTX132548 ICC/IF Image

Rab7A antibody detects Rab7A protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rab7A protein stained by Rab7A antibody (GTX132548) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX132548_42452_20160428_WB_18121410_529.jpg

GTX132548 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Rab7 antibody (GTX132548) diluted at 1:1000.

GTX132548_42452_20171115_ICC_IF_R_18121410_331.jpg

GTX132548 ICC/IF Image

RAB7A antibody detects RAB7A protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAB7A stained by RAB7A antibody (GTX132548) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX132548_42452_20160601_IHC-P_M_18121410_892.jpg

GTX132548 IHC-P Image

Rab7 antibody detects Rab7 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Rab7 antibody (GTX132548) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX132548_42452_20160601_IHC-P_R_18121410_700.jpg

GTX132548 IHC-P Image

Rab7 antibody detects Rab7 protein at cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
Rab7 antibody (GTX132548) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf