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RABGAP1 antibody [C2C3], C-term

Anti-RABGAP1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX115742
Anti-RABGAP1 antibody [C2C3], C-term used in Western Blot (WB). GTX115742
Anti-RABGAP1 antibody [C2C3], C-term used in Western Blot (WB). GTX115742
Anti-RABGAP1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115742

Cat. No. GTX115742

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RABGAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

IMR32 , Mouse brain

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RABGAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624549

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB GTPase activating protein 1 , GAPCENA , TBC1D11

Cellular Localization

Cytoplasm , cytosol , Centrosome

Database

Research Area

DATA IMAGES

Anti-RABGAP1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX115742

GTX115742 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using RABGAP1(GTX115742) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RABGAP1 antibody [C2C3], C-term used in Western Blot (WB). GTX115742

GTX115742 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX115742 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RABGAP1 antibody [C2C3], C-term used in Western Blot (WB). GTX115742

GTX115742 WB Image

Sample (30 μg of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX115742 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RABGAP1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115742

GTX115742 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using RABGAP1(GTX115742) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169