GeneTex
United States (US)

RABGAP1 antibody [C2C3], C-term

GTX115742_40296_IHC_18121410_870.jpg
GTX115742_40296_WB_M_brain_18121410_987.jpg
GTX115742_40296_WB_18121410_720.jpg
GTX115742_40296_IFA_18121410_444.jpg

Cat No. GTX115742

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , U87-MG , MCF-7 , mouse brain

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RABGAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rab Gtpase Activating Protein 1,Gapcena,Tbc1D11,Rabgap1

Cellular Localization

Cytoplasm , cytosol , Centrosome

Database

Research Area

DATA IMAGES
GTX115742_40296_IHC_18121410_870.jpg

GTX115742 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using RABGAP1(GTX115742) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115742_40296_WB_M_brain_18121410_987.jpg

GTX115742 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX115742 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115742_40296_WB_18121410_720.jpg

GTX115742 WB Image

Sample (30 µg of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX115742 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115742_40296_IFA_18121410_444.jpg

GTX115742 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using RABGAP1(GTX115742) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169