GeneTex
United States (US)

RABGGTB antibody

GTX105874_40737_WB_M_Brain_19011506_170.jpg
GTX105874_40737_20170518_WB_19011506_996.jpg
GTX105874_40737_IHC_19011506_631.jpg
GTX105874_40737_WB_R_Brain_19011506_427.jpg
GTX105874_40737_IFA_19011506_226.jpg

Cat No. GTX105874

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , Mouse brain , Rat brain

Predict Reactivity

Zebrafish, Sheep, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RABGGTB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GGTB antibody, RABGGTB antibody, rab GG transferase beta antibody, GGTase-II-beta antibody, rab geranyl-geranyltransferase subunit beta antibody, type II protein geranyl-geranyltransferase subunit beta antibody, rab geranylgeranyltransferase subunit beta antibody, rab GGTase beta antibody, geranylgeranyl transferase type II subunit beta antibody, geranylgeranyl transferase type-2 subunit beta antibody, "Rab geranylgeranyltransferase, beta subunit antibody"

Database

DATA IMAGES
GTX105874_40737_WB_M_Brain_19011506_170.jpg

GTX105874 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX105874 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105874_40737_20170518_WB_19011506_996.jpg

GTX105874 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RABGGTB antibody (GTX105874) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105874_40737_IHC_19011506_631.jpg

GTX105874 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using RABGGTB(GTX105874) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105874_40737_WB_R_Brain_19011506_427.jpg

GTX105874 WB Image

Sample (50 µg of whole cell lysate)
A: Rat brain
10% SDS PAGE
GTX105874 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105874_40737_IFA_19011506_226.jpg

GTX105874 ICC/IF Image

RABGGTB antibody detects RABGGTB protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RABGGTB protein stained by RABGGTB antibody (GTX105874) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169