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RAC2 antibody [N1C3]

Anti-RAC2 antibody [N1C3] used in Western Blot (WB). GTX113262
Anti-RAC2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113262
Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262
Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262
Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262

Cat. No. GTX113262

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Predict Reactivity

Rat, Zebrafish, Drosophila, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951589

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rac family small GTPase 2 , EN-7 , Gx , HSPC022 , p21-Rac2

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RAC2 antibody [N1C3] used in Western Blot (WB). GTX113262

GTX113262 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAC2 antibody [N1C3] (GTX113262) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAC2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113262

GTX113262 ICC/IF Image

RAC2 antibody [N1C3] detects RAC2 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MOLT-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAC2 stained by RAC2 antibody [N1C3] (GTX113262) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262

GTX113262 IHC-P Image

RAC2 antibody [N1C3] detects RAC2 protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
RAC2 antibody [N1C3] (GTX113262) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262

GTX113262 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using RAC2(GTX113262) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262

GTX113262 IHC-P Image

RAC2 antibody [N1C3] detects RAC2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
RAC2 stained by RAC2 antibody [N1C3] (GTX113262) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for RAC2 antibody [N1C3] (GTX113262). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for RAC2 antibody [N1C3] (GTX113262). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169