APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Zebrafish, Drosophila, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.43 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human RAC2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951589
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Rac family small GTPase 2 , EN-7 , Gx , HSPC022 , p21-Rac2
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262](/templates/default/js/lightbox/images/loading.gif "Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262")
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GTX113262 IHC-P Image
RAC2 antibody [N1C3] detects RAC2 protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
RAC2 antibody [N1C3] (GTX113262) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113262 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using RAC2(GTX113262) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113262 ICC/IF Image
RAC2 antibody [N1C3] detects RAC2 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: MOLT-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAC2 stained by RAC2 antibody [N1C3] (GTX113262) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX113262 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAC2 antibody [N1C3] (GTX113262) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113262 IHC-P Image
RAC2 antibody [N1C3] detects RAC2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.RAC2 stained by RAC2 antibody [N1C3] (GTX113262) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for RAC2 antibody [N1C3] (GTX113262). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RAC2 antibody [N1C3] (GTX113262). Be the first to share your experience with this product.
![Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262](/upload/website/prouct_img/small/GTX113262/GTX113262_40114_IHC_M_w_23060501_734.webp "Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262")
![Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262](/upload/website/prouct_img/small/GTX113262/GTX113262_40114_IHC_w_23060501_479.webp "Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262")
![Anti-RAC2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113262](/upload/website/prouct_img/small/GTX113262/GTX113262_44664_20230512_ICC_IF_23060622_205.webp "Anti-RAC2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113262")
![Anti-RAC2 antibody [N1C3] used in Western Blot (WB). GTX113262](/upload/website/prouct_img/small/GTX113262/GTX113262_44622_20220527_WB_22111518_171.webp "Anti-RAC2 antibody [N1C3] used in Western Blot (WB). GTX113262")
![Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262](/upload/website/prouct_img/small/GTX113262/GTX113262_44664_20230303_IHC-P_M_23032819_312.webp "Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262")
![Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262](/upload/website/prouct_img/normal/GTX113262/GTX113262_40114_IHC_M_w_23060501_734.webp "Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262")
![Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262](/upload/website/prouct_img/normal/GTX113262/GTX113262_40114_IHC_w_23060501_479.webp "Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262")
![Anti-RAC2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113262](/upload/website/prouct_img/normal/GTX113262/GTX113262_44664_20230512_ICC_IF_23060622_205.webp "Anti-RAC2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113262")
![Anti-RAC2 antibody [N1C3] used in Western Blot (WB). GTX113262](/upload/website/prouct_img/normal/GTX113262/GTX113262_44622_20220527_WB_22111518_171.webp "Anti-RAC2 antibody [N1C3] used in Western Blot (WB). GTX113262")
![Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262](/upload/website/prouct_img/normal/GTX113262/GTX113262_44664_20230303_IHC-P_M_23032819_312.webp "Anti-RAC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113262")