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RAC2 antibody

Anti-RAC2 antibody used in Western Blot (WB). GTX22244
Anti-RAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX22244
Anti-RAC2 antibody used in Western Blot (WB). GTX22244

Cat. No. GTX22244

Reference 3

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
50 μg ($399)
See all RAC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX22244-PEP).

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence PQPTRQQKRACSLL, from the C Terminus of the protein sequence according to NP_002863.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_370272

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rac family small GTPase 2 , EN-7 , Gx , HSPC022 , p21-Rac2

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the Ras superfamily of small guanosine triphosphate (GTP)-metabolizing proteins. The encoded protein localizes to the plasma membrane, where it regulates diverse processes, such as secretion, phagocytosis, and cell polarization. Activity of this protein is also involved in the generation of reactive oxygen species. Mutations in this gene are associated with neutrophil immunodeficiency syndrome. There is a pseudogene for this gene on chromosome 6. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-RAC2 antibody used in Western Blot (WB). GTX22244

GTX22244 WB Image

WB analysis of Molt-4 cell lysate using GTX22244 RAC2 antibody.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-RAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX22244

GTX22244 IHC-P Image

IHC-P analysis of human tonsil using GTX22244 RAC2 antibody.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 0.3μg/ml
A) Strong staining in the interfollicular area and mantle zone. B) Positive cells in the interfollicular area.

Anti-RAC2 antibody used in Western Blot (WB). GTX22244

GTX22244 WB Image

WB analysis of human thymus (A) and tonsil (B) lysate using GTX22244 RAC2 antibody.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Zou W et al. Inflamm Res 2012; 61 (8) : 853-62 AKT-mediated regulation of polarization in differentiated human neutrophil-like HL-60 cells.
Asai A et al. Biochem Biophys Res Commun 2011; 406 (2) : 273-7 Involvement of Rac GTPase activation in phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to ICAM-1.
Application : WB
Reactivity : Human
Niggli V et al. Biochem Biophys Res Commun 2009; 386 (4) : 688-92 Specific roles of Rac1 and Rac2 in motile functions of HT1080 fibrosarcoma cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399