APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 10μg/ml |
| 1μg/ml |
| Assay dependent |
|
Note :
ICC/IF
Strong expression of the protein seen in the cytoplasm of MCF7 cells.
IHC-P
Human Testis, shows nuclear staining of many cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX22270-PEP).
Product Note
This antibody is expected to recognize all reported isoforms (NP_037409.2; NP_001306934.1; NP_001306935.1; NP_001306936.1). Reported variants represent identical protein: NP_001306928.1, NP_001119576.1, NP_037409.2, NP_001306931.1, NP_001306929.1, NP_001119575.1, NP_001306932.1, NP_001306930.1, NP_001306933.1
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-GRQGNFFASPMLK, from the C Terminus of the protein sequence according to NP_037409.2; NP_001306934.1; NP_001306935.1; NP_001306936.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Rac GTPase activating protein 1 , CYK4 , HsCYK-4 , ID-GAP , MgcRacGAP
Cellular Localization
Nucleus , Cytoplasm
Background
This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016]
Database
Research Area
DATA IMAGES
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GTX22270 ICC/IF Image
ICC/IF analysis of PFA fixed MCF7 cells using GTX22270 RACGAP1 antibody.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
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GTX22270 IHC-P Image
IHC-P analysis of human testis using GTX22270 RACGAP1 antibody.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 4μg/ml
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GTX22270 FACS Image
FACS analysis of PFA fixed MCF7 cells using GTX22270 RACGAP1 antibody.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml
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GTX22270 WB Image
WB analysis of A431 nuclear (A), Jurkat (B), Jurkat nuclear (C) and negative control human pancreas (D) lysate using GTX22270 RACGAP1 antibody.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for RACGAP1 antibody (GTX22270). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RACGAP1 antibody (GTX22270). Be the first to share your experience with this product.
