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RACGAP1 antibody

Anti-RACGAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32831
Anti-RACGAP1 antibody used in Western Blot (WB). GTX32831

Cat No. GTX32831

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 353-632 of human RACGAP1 (NP_001119576.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rac GTPase activating protein 1 , CYK4 , HsCYK-4 , ID-GAP , MgcRacGAP

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-RACGAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32831

GTX32831 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX32831 RACGAP1 antibody.
Blue : DAPI

Anti-RACGAP1 antibody used in Western Blot (WB). GTX32831

GTX32831 WB Image

WB analysis of various samples using GTX32831 RACGAP1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 329