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RACGAP1 antibody

Anti-RACGAP1 antibody used in Western Blot (WB). GTX32831
Anti-RACGAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32831

Cat. No. GTX32831

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all RACGAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 353-632 of human RACGAP1 (NP_001119576.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rac GTPase activating protein 1 , CYK4 , HsCYK-4 , ID-GAP , MgcRacGAP

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-RACGAP1 antibody used in Western Blot (WB). GTX32831

GTX32831 WB Image

WB analysis of various sample lysates using GTX32831 RACGAP1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RACGAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32831

GTX32831 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32831 RACGAP1 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399