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RAD18 antibody

Orthogonal
Anti-RAD18 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00886
Anti-RAD18 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00886
Anti-RAD18 antibody used in Western Blot (WB). GTX00886

Cat. No. GTX00886

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μg ($399)
See all RAD18 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

GST-fusion protein containing 113 carboxyl terminal of human Rad18

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD18 E3 ubiquitin protein ligase , RAD18 , RAD18, E3 ubiquitin protein ligase , RNF73

Cellular Localization

Nucleus,Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome

Background

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RAD18 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00886

GTX00886 ICC/IF Image

Orthogonal

ICC/IF analysis of GM637 cells were irradiated with/without UV (15/m2) for 4 hrs using GTX00886 RAD18 antibody.
Fixation : Methanol
Dilution : 1:250

Anti-RAD18 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00886

GTX00886 ICC/IF Image

ICC/IF analysis of GM637 cells using GTX00886 RAD18 antibody. Rad18 protein is stained as yellow dots in nuclei.

Anti-RAD18 antibody used in Western Blot (WB). GTX00886

GTX00886 WB Image

WB analysis of A549 whole cell lysate using GTX00886 RAD18 antibody. The lower thick band is native Rad18 and the upper thin band is mono-ubiquitinated Rad18.
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399