APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
GST-fusion protein containing 113 carboxyl terminal of human Rad18
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
RAD18 E3 ubiquitin protein ligase , RAD18 , RAD18, E3 ubiquitin protein ligase , RNF73
Cellular Localization
Nucleus,Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome
Background
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX00886 ICC/IF Image
ICC/IF analysis of GM637 cells were irradiated with/without UV (15/m2) for 4 hrs using GTX00886 RAD18 antibody. Fixation : Methanol Dilution : 1:250
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GTX00886 ICC/IF Image
ICC/IF analysis of GM637 cells using GTX00886 RAD18 antibody. Rad18 protein is stained as yellow dots in nuclei.
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GTX00886 WB Image
WB analysis of A549 whole cell lysate using GTX00886 RAD18 antibody. The lower thick band is native Rad18 and the upper thin band is mono-ubiquitinated Rad18. Dilution : 1:1000
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REFERENCE
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REVIEW
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