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RAD18 antibody

Anti-RAD18 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00887
Anti-RAD18 antibody used in Western Blot (WB). GTX00887
Anti-RAD18 antibody used in IHC (Paraffin sections) (IHC-P). GTX00887

Cat No. GTX00887

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Mouse
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF Assay dependent
IHC-P 1:100-1:300
IP 1:200-1:500
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

GST-fusion protein containing 100 carboxyl terminal amino acids of mouse Rad18

Purification

Purified IgG
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Nucleus,Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome

Database

Research Area

DATA IMAGES

Anti-RAD18 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00887

GTX00887 ICC/IF Image

ICC/IF analysis of Wild-type (+/+) and RAD18 knock-out mouse ES cells (-/-) using GTX00887 RAD18 antibody.
Prefixed 4% PFA and fixed with 80% methanol

Anti-RAD18 antibody used in Western Blot (WB). GTX00887

GTX00887 WB Image

WB analysis of WT and RAD18-/- knock-out mouse ES cells using GTX00887 RAD18 antibody.

Anti-RAD18 antibody used in IHC (Paraffin sections) (IHC-P). GTX00887

GTX00887 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX00887 RAD18 antibody. Arrows indicate undifferentiated spermatogonia. Signals were enhanced with TSA plus biotin system and detected by DAB substrate.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 369