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RAD18 antibody

KO/KD
Anti-RAD18 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00887
Anti-RAD18 antibody used in IHC (Paraffin sections) (IHC-P). GTX00887
Anti-RAD18 antibody used in Western Blot (WB). GTX00887

Cat. No. GTX00887

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Mouse
Package
100 μg ($399)
See all RAD18 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
IHC-P 1:100-1:300
IP 1:200-1:500
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

GST-fusion protein containing 100 carboxyl terminal amino acids of mouse Rad18

Purification

Purified IgG
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Nucleus,Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome

Database

Research Area

DATA IMAGES

Anti-RAD18 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00887

GTX00887 ICC/IF Image

KO/KD

ICC/IF analysis of Wild-type (+/+) and RAD18 knock-out mouse ES cells (-/-) using GTX00887 RAD18 antibody.
Prefixed 4% PFA and fixed with 80% methanol

Anti-RAD18 antibody used in IHC (Paraffin sections) (IHC-P). GTX00887

GTX00887 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX00887 RAD18 antibody. Arrows indicate undifferentiated spermatogonia. Signals were enhanced with TSA plus biotin system and detected by DAB substrate.

Anti-RAD18 antibody used in Western Blot (WB). GTX00887

GTX00887 WB Image

KO/KD

WB analysis of WT and RAD18-/- knock-out mouse ES cells using GTX00887 RAD18 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399