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RAD23A antibody

Anti-RAD23A antibody used in Western Blot (WB). GTX23836

Cat No. GTX23836

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human
Package
50 μg ($309)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ELISA 1:4000 - 1:16000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: KSPSEESAPTTSPEC, corresponding to amino acids 122-135 of Human hHR23A.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAD23 homolog A, nucleotide excision repair protein , HHR23A , HR23A

Cellular Localization

Nucleus

Background

HHR23A was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Database

Research Area

DATA IMAGES

Anti-RAD23A antibody used in Western Blot (WB). GTX23836

GTX23836 WB Image

Western blot showing GeneTex's Affinity Purified anti-HR23A antibody detects endogenous human HR23A. Reactivity is shown against HeLa nuclear extract (lane 1) and HeLa (lane 2), A431 (lane 3), Jurkat (lane 4) and 293 whole cell lysates (lane 5). Comparison to a molecular weight marker (at left) indicates a band of ~60 kDa corresponding to HR23A. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using HRP conjugated Rb-a-Goat IgG and chemilumi-nescence reagent with a 30-min exposure time. Other detection systems will yield similar results.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 309