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RAD23B antibody [5H1-A10-A7]

Anti-RAD23B antibody [5H1-A10-A7] used in Western Blot (WB). GTX16485
Anti-RAD23B antibody [5H1-A10-A7] used in Western Blot (WB). GTX16485
Anti-RAD23B antibody [5H1-A10-A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16485
Anti-RAD23B antibody [5H1-A10-A7] used in IHC (Paraffin sections) (IHC-P). GTX16485

Cat. No. GTX16485

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5H1-A10-A7

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Hamster, Monkey
Package
100 μl ($399)
See all RAD23B products

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:1000.
For ICC/IF: Use at a dilution of 1:100.
For IHC-P: Use at an assay depeendent dilution.
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human RAD23B protein fragments expressed in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD23 homolog B, nucleotide excision repair protein , HHR23B , HR23B , P58

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-RAD23B antibody [5H1-A10-A7] used in Western Blot (WB). GTX16485

GTX16485 WB Image

WB analysis of various cell lysates using hHR23b antibody [5H1-A10-A7] at a dilution of 1:1000.

Anti-RAD23B antibody [5H1-A10-A7] used in Western Blot (WB). GTX16485

GTX16485 WB Image

WB analysis of various cell lysates using hHR23b antibody [5H1-A10-A7] at a dilution of 1:1000.

Anti-RAD23B antibody [5H1-A10-A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16485

GTX16485 ICC/IF Image

ICC/IF analysis of HeLa cells using hHR23b antibody [5H1-A10-A7] at a dilution of 1:100.

Anti-RAD23B antibody [5H1-A10-A7] used in IHC (Paraffin sections) (IHC-P). GTX16485

GTX16485 IHC-P Image

IHC-P analysis of prostate cancer using hHR23b antibody [5H1-A10-A7] at a dilution of 1:100. Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399