APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000 |
1:50-1:200 |
1:50-1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.2, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant human Rad23B protein fragments expressed in E.coli.
Purification
Purified by antigen-affinity chromatography
Purity
> 95% (by SDS-PAGE)
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
RAD23 homolog B, nucleotide excision repair protein , HHR23B , HR23B , P58
Cellular Localization
Nucleus,Cytoplasm
Background
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011]
Database
Research Area
DATA IMAGES
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GTX66845 WB Image
WB analysis of K562, SW480, CHO-K1, 3T3, and COS7 cell lysates using GTX66845 RAD23B antibody [5H1]. Dilution : 1:1000
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GTX66845 IHC-P Image
IHC-P analysis of prostate cancer tissue using GTX66845 RAD23B antibody [5H1]. Antigen retrieval : citrate buffer (pH 6.0)
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GTX66845 ICC/IF Image
ICC/IF analysis of 4% PFA-fixed HeLa cells using GTX66845 RAD23B antibody [5H1]. Dilution : 1:100
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REFERENCE
There are currently no references for RAD23B antibody [5H1] (GTX66845). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RAD23B antibody [5H1] (GTX66845). Be the first to share your experience with this product.