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RAD23B antibody [5H1]

Anti-RAD23B antibody [5H1] used in Western Blot (WB). GTX66845
Anti-RAD23B antibody [5H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66845
Anti-RAD23B antibody [5H1] used in IHC (Paraffin sections) (IHC-P). GTX66845

Cat No. GTX66845

Host

Mouse

Clonality

Monoclonal

Clone Name

5H1

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Hamster, Monkey
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human Rad23B protein fragments expressed in E.coli.

Purification

Purified by antigen-affinity chromatography

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAD23 homolog B, nucleotide excision repair protein , HHR23B , HR23B , P58

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-RAD23B antibody [5H1] used in Western Blot (WB). GTX66845

GTX66845 WB Image

WB analysis of K562, SW480, CHO-K1, 3T3, and COS7 cell lysates using GTX66845 RAD23B antibody [5H1].
Dilution : 1:1000

Anti-RAD23B antibody [5H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66845

GTX66845 ICC/IF Image

ICC/IF analysis of 4% PFA-fixed HeLa cells using GTX66845 RAD23B antibody [5H1].
Dilution : 1:100

Anti-RAD23B antibody [5H1] used in IHC (Paraffin sections) (IHC-P). GTX66845

GTX66845 IHC-P Image

IHC-P analysis of prostate cancer tissue using GTX66845 RAD23B antibody [5H1].
Antigen retrieval : citrate buffer (pH 6.0)

REFERENCE

There are currently no references for RAD23B antibody [5H1] (GTX66845). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for RAD23B antibody [5H1] (GTX66845). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399