RAD23B antibody

Cat. No. GTX103424

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all RAD23B products

Datasheet
Document

View product citations for antibody GTX103424 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , A431

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAD23B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171916

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD23 homolog B, nucleotide excision repair protein , HHR23B , HR23B , P58

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. [provided by RefSeq]

Database

Gene ID: 5887 RAD23B
UniProt: P54727 RAD23B

Research Area

DATA IMAGES

GTX103424 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
12% SDS PAGE
GTX103424 diluted at 1:10000

GTX103424 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using RAD23B(GTX103424) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103424 ICC/IF Image

RAD23B antibody detects RAD23B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAD23B protein stained by RAD23B antibody (GTX103424) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty