Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAD23B antibody (GTX103424) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

RAD23B antibody detects RAD23B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAD23B protein stained by RAD23B antibody (GTX103424) diluted at 1:500.
Blue: Hoechst 33343 staining.

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using RAD23B(GTX103424) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
12% SDS PAGE
GTX103424 diluted at 1:10000