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RAD23B antibody

Orthogonal
Anti-RAD23B antibody used in Western Blot (WB). GTX103424
Anti-RAD23B antibody used in Western Blot (WB). GTX103424
Anti-RAD23B antibody used in IHC (Paraffin sections) (IHC-P). GTX103424
Anti-RAD23B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103424

Cat. No. GTX103424

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RAD23B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , A431

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAD23B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171916

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD23 homolog B, nucleotide excision repair protein , HHR23B , HR23B , P58

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RAD23B antibody used in Western Blot (WB). GTX103424

GTX103424 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAD23B antibody (GTX103424) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-RAD23B antibody used in Western Blot (WB). GTX103424

GTX103424 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
12% SDS PAGE
GTX103424 diluted at 1:10000

Anti-RAD23B antibody used in IHC (Paraffin sections) (IHC-P). GTX103424

GTX103424 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using RAD23B(GTX103424) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAD23B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103424

GTX103424 ICC/IF Image

RAD23B antibody detects RAD23B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAD23B protein stained by RAD23B antibody (GTX103424) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169