GeneTex

  • Country / Location Selection

United States (US)

RAD23B antibody

Anti-RAD23B antibody used in (). GTX48751
Anti-RAD23B antibody used in Western Blot (WB). GTX48751
Anti-RAD23B antibody used in Western Blot (WB). GTX48751

Cat. No. GTX48751

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human
Package
50 μg ($399)
See all RAD23B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:2000-1:10000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to an internal region near aa 155-180 of human HR23B protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD23 homolog B, nucleotide excision repair protein , HHR23B , HR23B , P58

Cellular Localization

Nucleus,Cytoplasm

Background

HR23B (also known as UV excision repair protein RAD23 homolog B, XP-C repair complementing complex 58 kDa protein and p58) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Database

Research Area

DATA IMAGES

Anti-RAD23B antibody used in (). GTX48751

GTX48751 Image

Anti-RAD23B antibody used in Western Blot (WB). GTX48751

GTX48751 WB Image

Western blot using GeneTex's affinity purified anti-HR23B antibody shows detection of a band at ~58 kDa (arrowhead) corresponding to HR23B present in a HeLa whole cell lysate. Pre-incubation of antibody with immunizing peptide completely blocks reactivity (data not shown). Approximately 33 μg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane was probed overnight at 4ºC with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:20,000 dilution of IRDye800 conjugated rabbit anti-Goat IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red). IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Anti-RAD23B antibody used in Western Blot (WB). GTX48751

GTX48751 WB Image

WB analysis of HeLa whole cell lysate using GTX48751 RAD23B antibody.
Loading : 33 μg
Dilution : 1:500

REFERENCE

There are currently no references for RAD23B antibody (GTX48751). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for RAD23B antibody (GTX48751). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399