RAD51AP1 antibody [N1C1]

Name: RAD51AP1 antibody [N1C1]
Brand: GeneTex
SKU: GTX115455
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-RAD51AP1 antibody [N1C1] used in Western Blot (WB). GTX115455

Cat. No. GTX115455

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all RAD51AP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000

Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAD51AP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729638

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAD51 associated protein 1 , PIR51

Background

May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Functionally cooperates with PALB2 in promoting of D-loop formation by RAD51. Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA.

Database

Gene ID: 10635 RAD51AP1
UniProt: Q96B01 RAD51AP1

Research Area

DATA IMAGES

GTX115455 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with RAD51AP1 antibody [N1C1] (GTX115455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX115455 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAD51AP1 antibody [N1C1] (GTX115455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Bruno Silva et al. Nat Commun 2021; 12 (1) : 3760 TERRA transcription destabilizes telomere integrity to initiate break-induced replication in human ALT cells.

Application : WB

Reactivity : Human

Park SH et al. Nat Commun 2019; 10 (1) : 5718 ATAD5 promotes replication restart by regulating RAD51 and PCNA in response to replication stress.

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REVIEW