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RAD54B antibody

Anti-RAD54B antibody used in IHC (Paraffin sections) (IHC-P). GTX103291
Anti-RAD54B antibody used in Western Blot (WB). GTX103291
Anti-RAD54B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103291
Anti-RAD54B antibody used in Western Blot (WB). GTX103291

Cat No. GTX103291

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

Molt-4

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RAD54B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAD54 homolog B , RDH54

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RAD54B antibody used in IHC (Paraffin sections) (IHC-P). GTX103291

GTX103291 IHC-P Image

Rad54B antibody detects RAD54B protein at on SAS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SAS xenograft.
Rad54B antibody (GTX103291) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAD54B antibody used in Western Blot (WB). GTX103291

GTX103291 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX103291 diluted at 1:500

Anti-RAD54B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103291

GTX103291 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAD54B(GTX103291) antibody at 1:100 dilution.

Anti-RAD54B antibody used in Western Blot (WB). GTX103291

GTX103291 WB Image

The WB analysis of RAD54B antibody was published by Chen YJ and colleagues in the journal Sci Rep in 2017.PMID: 28630472

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169