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RAD54B antibody

Anti-RAD54B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103291
Anti-RAD54B antibody used in IHC (Paraffin sections) (IHC-P). GTX103291
Anti-RAD54B antibody used in Western Blot (WB). GTX103291
Anti-RAD54B antibody used in Western Blot (WB). GTX103291
Anti-RAD54B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103291

Cat. No. GTX103291

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RAD54B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

Molt-4

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RAD54B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951608

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD54 homolog B , RDH54

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RAD54B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103291

GTX103291 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAD54B(GTX103291) antibody at 1:100 dilution.

Anti-RAD54B antibody used in IHC (Paraffin sections) (IHC-P). GTX103291

GTX103291 IHC-P Image

Rad54B antibody detects RAD54B protein at on SAS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SAS xenograft.
Rad54B antibody (GTX103291) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAD54B antibody used in Western Blot (WB). GTX103291

GTX103291 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX103291 diluted at 1:500

Anti-RAD54B antibody used in Western Blot (WB). GTX103291

GTX103291 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28630472

Anti-RAD54B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103291

GTX103291 ICC/IF Image

The data was published in the journal J Virol in 2017.PMID: 28855246

REFERENCE

Application Reference

Hollingworth R et al. J Virol 2017; 91 (22) Localization of Double-Strand Break Repair Proteins to Viral Replication Compartments following Lytic Reactivation of Kaposi's Sarcoma-Associated Herpesvirus.
Chen YJ et al. Sci Rep 2017; 7 (1) : 3842 SMYD3 Promotes Homologous Recombination via Regulation of H3K4-mediated Gene Expression.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169