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RAD54L antibody [60.1]

Anti-RAD54L antibody [60.1] used in Western Blot (WB). GTX70306
Anti-RAD54L antibody [60.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70306
Anti-RAD54L antibody [60.1] used in Western Blot (WB). GTX70306
Anti-RAD54L antibody [60.1] used in Western Blot (WB). GTX70306

Cat. No. GTX70306

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 60.1

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all RAD54L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide - Combination of N & C peptide - Corresponding to a range between amino acids 1-300 and 450-741

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_377593

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD54 like , HR54 , RAD54A , hHR54 , hRAD54

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-RAD54L antibody [60.1] used in Western Blot (WB). GTX70306

GTX70306 WB Image

Detection of human Rad54 protein using GeneTex Rad54 60.1 monoclonal antibody (GTX70306) in Raji whole cell extract.

Anti-RAD54L antibody [60.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70306

GTX70306 ICC/IF Image

RAD54L antibody [60.1] detects RAD54L protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAD54L stained by RAD54L antibody [60.1] (GTX70306) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-RAD54L antibody [60.1] used in Western Blot (WB). GTX70306

GTX70306 WB Image

Anti-RAD54L antibody [60.1] used in Western Blot (WB). GTX70306

GTX70306 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RAD54L antibody [60.1] (GTX70306) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations