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RAD9A antibody [7B11]

Overexpression
Anti-RAD9A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83736
Anti-RAD9A antibody [7B11] used in Western Blot (WB). GTX83736
Anti-RAD9A antibody [7B11] used in Western Blot (WB). GTX83736
Anti-RAD9A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83736
Anti-RAD9A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83736
Anti-RAD9A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83736
Anti-RAD9A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83736

Cat. No. GTX83736

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7B11

Isotype

 IgG2a

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($519)
See all Rad9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
IHC-P 1:50
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human RAD9A expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD9 checkpoint clamp component A , RAD9

Cellular Localization

Nucleus

Background

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds | endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths | and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Database

Research Area

DATA IMAGES

Anti-RAD9A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83736

GTX83736 IHC-P Image

IHC-P analysis of colon tissue using GTX83736 RAD9A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-RAD9A antibody [7B11] used in Western Blot (WB). GTX83736

GTX83736 WB Image

Overexpression

WB analysis of HEK293T cells transfected with RAD9A plasmid (Right) or empty vector (Left) for 48 hrs using GTX83736 RAD9A antibody [7B11].
Loading : 5 ug per lane

Anti-RAD9A antibody [7B11] used in Western Blot (WB). GTX83736

GTX83736 WB Image

WB analysis of various cell lines using GTX83736 RAD9A antibody [7B11].
Loading : 35 ug per lane

Anti-RAD9A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83736

GTX83736 IHC-P Image

IHC-P analysis of lung carcinoma tissue using GTX83736 RAD9A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-RAD9A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83736

GTX83736 IHC-P Image

IHC-P analysis of pancreas carcinoma tissue using GTX83736 RAD9A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-RAD9A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83736

GTX83736 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX83736 RAD9A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-RAD9A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83736

GTX83736 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX83736 RAD9A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519