*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HeLaS3 , Molt-4 , Raji
Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RAE1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Ribonucleic Acid Export 1,Mig14,Mrnp41,Mnrp41,Dj481F12.3,Dj800J21.1,Rae1
Cytoplasm , Nucleus
Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
GTX106289 ICC/IF Image
RAE1 antibody [C3], C-term detects RAE1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAE1 protein stained by RAE1 antibody [C3], C-term (GTX106289) diluted at 1:500.
Blue: Hoechst 33342 staining.
GTX106289 WB Image
Sample(30 μg of whole cell lysate)
12% SDS PAGE
GTX106289 diluted at 1:1500